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Cited by 528

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Counting Cytokinesis Proteins Globally and Locally in Fission Yeast

Wu, Jian-Qiu, Pollard, Thomas D.·2005·Science
DOI 10.1126/science.1113230
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Abstract

We used fluorescence microscopy to measure global and local concentrations of 28 cytoskeletal and signaling proteins fused to yellow fluorescent protein (YFP) in the fission yeast Schizosaccharomyces pombe . Native promoters controlled the expression of these functional YFP fusion proteins. Fluorescence measured by microscopy or flow cytometry was directly proportional to protein concentration measured by quantitative immunoblotting. Global cytoplasmic concentrations ranged from 0.04 (formin Cdc12p) to 63 micromolar (actin). Proteins concentrated up to 100 times in contractile rings and 7500 times in spindle pole bodies at certain times in the cell cycle. This approach can be used to measure the global and local concentrations of any fusion protein.

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Crossref · 10.1126/science.1113230

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  • CRISPR-Cas9 gene-editing technologies provide a promising alternative to overcome potential pitfalls of GFP-LC3 overexpression by generating knockin cell lines in which the coding sequence of GFP is added in frame with the 5' sequence of endogenous LC3.

    “Finally, a recent advent of CRISPR-Cas9 gene-editing technologies provides a promising alternative to overcome potential pitfalls of GFP-LC3 overexpression-the generation of knockin cell lines, in which the coding sequence of GFP is added in frame with the 5' sequence (encoding the Nterminal part) of endogenous LC3 [122,407].”

    From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [407]

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Metadata

Authors
Wu, J.-Q., & Pollard, T. D.
Affiliations
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520–8103, USA.
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2005-10-14
Volume
310
Issue
5746
Pages
310-314
ISSN
  • 0036-8075
  • 1095-9203
DOI
10.1126/science.1113230
Cited by
528
References
1
Language
en

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