The DOI matches its Crossref record.
Complete Replication of Hepatitis C Virus in Cell Culture
Abstract
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10 5 infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-α and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.
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Citations
Cited by
The HCV Life Cycle: In vitro Tissue Culture Systems and Therapeutic Targets
Gerold, G., & Pietschmann, T. (2014) · Digestive Diseases · doi:10.1159/000360830 · Cited by 4,854
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus
Meylan, E., Curran, J., Hofmann, K., Moradpour, D., Binder, M., Bartenschlager, R., & Tschopp, J. (2005) · Nature · doi:10.1038/nature04193 · Cited by 2,401
Mechanism of action of interferon and ribavirin in treatment of hepatitis C
Feld, J. J., & Hoofnagle, J. H. (2005) · Nature · doi:10.1038/nature04082 · Cited by 992
Marcello, T., Grakoui, A., Barba–Spaeth, G., Machlin, E. S., Kotenko, S. V., Macdonald, M. R., & Rice, C. M. (2006) · Gastroenterology · doi:10.1053/j.gastro.2006.09.052 · Cited by 561
Self-citationAppel, N., Zayas, M., Miller, S., Krijnse-Locker, J., Schaller, T., Friebe, P., Kallis, S., Engel, U., & Bartenschlager, R. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000035 · Cited by 466
Metadata
- Authors
- Lindenbach, B. D., Evans, M. J., Syder, A. J., Wölk, B., Tellinghuisen, T. L., Liu, C. C., Maruyama, T., Hynes, R. O., Burton, D. R., McKeating, J. A., & Rice, C. M.
- Venue
- Science
- Published
- 2005-07-22
- Volume
- 309
- Issue
- 5734
- Pages
- 623-626
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2147126871
- Cited by
- 2,238
- References
- 0
- FWCI
- 104.47
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lindenbach, B. D., Evans, M. J., Syder, A. J., Wölk, B., Tellinghuisen, T. L., Liu, C. C., Maruyama, T., Hynes, R. O., Burton, D. R., McKeating, J. A., & Rice, C. M. (2005). Complete Replication of Hepatitis C Virus in Cell Culture. Science. https://doi.org/10.1126/science.1114016
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
