MicroRNA Expression in Zebrafish Embryonic Development
Abstract
MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid–modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, which suggests that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
miR-140-5p and miR-140-3p are abundantly and relatively specifically expressed in chondrocytes.
“miR-140-5p and miR-140-3p (collectively miR-140) are abundantly and relatively specifically expressed in chondrocytes14,15”
From Gain-of-function mutation of microRNA-140 in human skeletal dysplasia (2019) · cites this paper as [14]
Previous studies had compared miRNA expression in whole brain tissue with other tissues or assessed differences during development of individual neural cell types.
“Previous studies had instead compared the miRNA expression in whole brain tissue with the expression in other tissues of the same organism (Sempere et al., 2004;Wienholds et al., 2005) or assessed differences in miRNA expression during different stages of development of individual neural cell types (Krichevsky et al., 2003;Smirnova et al., 2005;Zhao et al., 2010).”
From Comprehensive expression analyses of neural cell-type-specific miRNAs identify new determinants of the specification and maintenance of neuronal phenotypes (2013) · cites this paper as Wienholds et al., 2005
No citation lists for this paper
No citation lists for this paper yet — checked again daily.
References 8 · Cited by 1,551
- Authors
- Wienholds, E., Kloosterman, W. P., Miska, E., Alvarez-Saavedra, E., Berezikov, E., de Bruijn, E., Horvitz, H. R., Kauppinen, S., & Plasterk, R. H. A.
- Affiliations
- University of Copenhagen (DK)
- Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, the Netherlands.
- Corresponding author
- Plasterk, Ronald H. A.
- ORCID
- Venue
- Science
- Published
- 2005-07-08
- Volume
- 309
- Issue
- 5732
- Pages
- 310-311
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1981186391
- Cited by
- 1,551
- References
- 8
- Citations by year
- 2026: 9 · 2025: 19 · 2024: 26 · 2023: 31 · 2022: 48 · 2021: 53 · 2020: 63 · 2019: 65 · 2018: 70 · 2017: 78 · 2016: 72 · 2015: 69 · 2014: 104 · 2013: 111 · 2012: 114
- FWCI
- 37.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Keywords
- microRNA expression, zebrafish embryos, embryonic development, tissue-specific gene expression, gene expression regulation, histogenesis
- MeSH terms
- Animals, Embryo, Nonmammalian, Multigene Family, Organ Specificity, Time Factors, Zebrafish, Blotting, Northern, Oligonucleotide Probes, Gene Expression, In Situ Hybridization, Oligonucleotide Array Sequence Analysis, MicroRNAs, Embryonic Development
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
