The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Emergent Properties of Reduced-Genome Escherichia coli
Abstract
With the use of synthetic biology, we reduced the Escherichia coli K-12 genome by making planned, precise deletions. The multiple-deletion series (MDS) strains, with genome reductions up to 15%, were designed by identifying nonessential genes and sequences for elimination, including recombinogenic or mobile DNA and cryptic virulence genes, while preserving good growth profiles and protein production. Genome reduction also led to unanticipated beneficial properties: high electroporation efficiency and accurate propagation of recombinant genes and plasmids that were unstable in other strains. Eradication of stress-induced transposition evidently stabilized the MDS genomes and provided some of the new properties.
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Cites
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Design and synthesis of a minimal bacterial genome
Hutchison, C. A., Chuang, R.-Y., Noskov, V. N., Assad-Garcia, N., Deerinck, T. J., Ellisman, M. H., Gill, J., Kannan, K., Karas, B. J., Ma, L., et al. (2016) · Science · doi:10.1126/science.aad6253 · Cited by 1,500
Expanding and Reprogramming the Genetic Code of Cells and Animals
Chin, J. W. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035737 · Cited by 476
Biotechnological domestication of pseudomonads using synthetic biology
Nikel, P. I., Martínez-García, E., & de Lorenzo, V. (2014) · Nature Reviews Microbiology · doi:10.1038/nrmicro3253 · Cited by 381
Biofuel alternatives to ethanol: pumping the microbial well
Fortman, J. L., Chhabra, S., Mukhopadhyay, A., Chou, H., Lee, T. S., Steen, E., & Keasling, J. D. (2008) · Trends in Biotechnology · doi:10.1016/j.tibtech.2008.03.008 · Cited by 366
Genome‐scale engineering for systems and synthetic biology
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Metadata
- Authors
- Pósfai, G., Plunkett, G., Fehér, T., Frisch, D., Keil, G. M., Umenhoffer, K., Kolisnychenko, V., Stahl, B., Sharma, S. S., de Arruda, M., Burland, V., Harcum, S. W., & Blattner, F. R.
- Venue
- Science
- Published
- 2006-05-19
- Volume
- 312
- Issue
- 5776
- Pages
- 1044-1046
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2159916488
- Cited by
- 697
- References
- 14
- FWCI
- 11.30
- Type
- journal article
- Language
- en
- Open access
- closed
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- Pósfai, G., Plunkett, G., Fehér, T., Frisch, D., Keil, G. M., Umenhoffer, K., Kolisnychenko, V., Stahl, B., Sharma, S. S., de Arruda, M., Burland, V., Harcum, S. W., & Blattner, F. R. (2006). Emergent Properties of Reduced-Genome Escherichia coli. Science. https://doi.org/10.1126/science.1126439
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