The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria
Abstract
Gene inventory and metagenomic techniques have allowed rapid exploration of bacterial diversity and the potential physiologies present within microbial communities. However, it remains nontrivial to discover the identities of environmental bacteria carrying two or more genes of interest. We have used microfluidic digital polymerase chain reaction (PCR) to amplify and analyze multiple, different genes obtained from single bacterial cells harvested from nature. A gene encoding a key enzyme involved in the mutualistic symbiosis occurring between termites and their gut microbiota was used as an experimental hook to discover the previously unknown ribosomal RNA–based species identity of several symbionts. The ability to systematically identify bacteria carrying a particular gene and to link any two or more genes of interest to single species residing in complex ecosystems opens up new opportunities for research on the environment.
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Cites
Environmental genome shotgun sequencing of the sargasso sea
Venter, J. C., Remington, K., Heidelberg, J. F., Halpern, A. L., Rusch, D., Eisen, J. A., Wu, D., Paulsen, I., Nelson, K. E., Nelson, W., et al. (2004) · Science · doi:10.1126/science.1093857 · Cited by 4,376
Community structure and metabolism through reconstruction of microbial genomes from the environment
Tyson, G. W., Chapman, J., Hugenholtz, P., Allen, E. E., Ram, R. J., Richardson, P. M., Solovyev, V. V., Rubin, E. M., Rokhsar, D. S., & Banfield, J. F. (2004) · Nature · doi:10.1038/nature02340 · Cited by 2,417
Vogelstein, B., & Kinzler, K. W. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.16.9236 · Cited by 1,770
Cited by
High-throughput droplet digital PCR system for absolute quantitation of DNA copy number
Hindson, B. J., Ness, K. D., Masquelier, D. A., Belgrader, P., Heredia, N. J., Makarewicz, A. J., Bright, I. J., Lucero, M. Y., Hiddessen, A. L., Legler, T. C., et al. (2011) · Analytical Chemistry · doi:10.1021/ac202028g · Cited by 2,792
Twenty-Five Years of Quantitative PCR for Gene Expression Analysis
VanGuilder, H. D., Vrana, K. E., & Freeman, W. M. (2008) · BioTechniques · doi:10.2144/000112776 · Cited by 1,246
Detection and quantification of rare mutations with massively parallel sequencing
Kinde, I., Wu, J., Papadopoulos, N., Kinzler, K. W., & Vogelstein, B. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1105422108 · Cited by 1,144
Microfabrication meets microbiology
Weibel, D. B., DiLuzio, W. R., & Whitesides, G. M. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1616 · Cited by 788
Quan, P.-L., Sauzade, M., & Brouzes, E. (2018) · Sensors · doi:10.3390/s18041271 · Cited by 664
Metadata
- Authors
- Ottesen, E. A., Hong, J. W., Quake, S. R., & Leadbetter, J. R.
- Venue
- Science
- Published
- 2006-12
- Volume
- 314
- Issue
- 5804
- Pages
- 1464-1467
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1984857185
- Cited by
- 737
- References
- 3
- FWCI
- 30.55
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ottesen, E. A., Hong, J. W., Quake, S. R., & Leadbetter, J. R. (2006). Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria. Science. https://doi.org/10.1126/science.1131370
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