The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
N-Linked Glycosylation of Folded Proteins by the Bacterial Oligosaccharyltransferase
Abstract
N-linked protein glycosylation is found in all domains of life. In eukaryotes, it is the most abundant protein modification of secretory and membrane proteins, and the process is coupled to protein translocation and folding. We found that in bacteria, N-glycosylation can occur independently of the protein translocation machinery. In an in vitro assay, bacterial oligosaccharyltransferase glycosylated a folded endogenous substrate protein with high efficiency and folded bovine ribonuclease A with low efficiency. Unfolding the eukaryotic substrate greatly increased glycosylation. We propose that in the bacterial system, glycosylation sites are located in flexible parts of folded proteins, whereas the eukaryotic cotranslational glycosylation evolved to a mechanism presenting the substrate in a flexible form before folding.
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Citations
Cites
Roles of N-Linked Glycans in the Endoplasmic Reticulum
Helenius, A., & Aebi, M. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.073752 · Cited by 2,037
Self-citationAnti-Inflammatory Activity of Immunoglobulin G Resulting from Fc Sialylation
Kaneko, Y., Nimmerjahn, F., & Ravetch, J. V. (2006) · Science · doi:10.1126/science.1129594 · Cited by 1,754
Glycosylation and the Immune System
Rudd, P. M., Elliott, T., Cresswell, P., Wilson, I. A., & Dwek, R. A. (2001) · Science · doi:10.1126/science.291.5512.2370 · Cited by 1,588
Cited by
Mechanisms and principles of N-linked protein glycosylation
Schwarz, F., & Aebi, M. (2011) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2011.08.005 · Cited by 695
Self-citationProtein glycosylation in bacteria: sweeter than ever
Nothaft, H., & Szymanski, C. M. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2383 · Cited by 530
Ruiz-Canada, C., Kelleher, D. J., & Gilmore, R. (2009) · Cell · doi:10.1016/j.cell.2008.11.047 · Cited by 391
N-Linked Protein Glycosylation in the Endoplasmic Reticulum
Breitling, J., & Aebi, M. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a013359 · Cited by 332
Self-citationMoradi, S. V., Hussein, W. M., Varamini, P., Simerska, P., & Toth, I. (2016) · Chemical Science · doi:10.1039/c5sc04392a · Cited by 286
Metadata
- Authors
- Kowarik, M., Numao, S., Feldman, M. F., Schulz, B. L., Callewaert, N., Kiermaier, E., Catrein, I., & Aebi, M.
- Venue
- Science
- Published
- 2006-11-17
- Volume
- 314
- Issue
- 5802
- Pages
- 1148-1150
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2078703243
- Cited by
- 221
- References
- 3
- FWCI
- 5.03
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kowarik, M., Numao, S., Feldman, M. F., Schulz, B. L., Callewaert, N., Kiermaier, E., Catrein, I., & Aebi, M. (2006). N-Linked Glycosylation of Folded Proteins by the Bacterial Oligosaccharyltransferase. Science. https://doi.org/10.1126/science.1134351
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