The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Ubiquitin-Like Protein Involved in the Proteasome Pathway of Mycobacterium tuberculosis
Open accessAbstract
The protein modifier ubiquitin is a signal for proteasome-mediated degradation in eukaryotes. Proteasome-bearing prokaryotes have been thought to degrade proteins via a ubiquitin-independent pathway. We have identified a prokaryotic ubiquitin-like protein, Pup (Rv2111c), which was specifically conjugated to proteasome substrates in the pathogen Mycobacterium tuberculosis . Pupylation occurred on lysines and required proteasome accessory factor A (PafA). In a pafA mutant, pupylated proteins were absent and substrates accumulated, thereby connecting pupylation with degradation. Although analogous to ubiquitylation, pupylation appears to proceed by a different chemistry. Thus, like eukaryotes, bacteria may use a small-protein modifier to control protein stability.
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Cited by
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Intrinsically disordered proteins: a 10-year recap
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Ubiquitin-like protein conjugation and the ubiquitin–proteasome system as drug targets
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“Because the prokaryotic ubiquitin-like protein (Pup)proteasome system of Mtb is essential for pathogenesis 88, 89 , the various components of the system present excellent opportunities for therapeutic intervention.”
Background
Metadata
- Authors
- Pearce, M. J., Mintseris, J., Ferreyra, J., Gygi, S. P., & Darwin, K. H.
- Venue
- Science
- Published
- 2008-11-14
- Volume
- 322
- Issue
- 5904
- Pages
- 1104-1107
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2081444617
- Cited by
- 377
- References
- 21
- FWCI
- 11.04
- Type
- journal article
- Language
- en
- Open access
- green
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- Pearce, M. J., Mintseris, J., Ferreyra, J., Gygi, S. P., & Darwin, K. H. (2008). Ubiquitin-Like Protein Involved in the Proteasome Pathway of Mycobacterium tuberculosis. Science. https://doi.org/10.1126/science.1163885
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
