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Cited by 148Developmental Biology and Gene RegulationCellular Mechanics and InteractionsCell Image Analysis Techniques

Dynamic Analyses of Drosophila Gastrulation Provide Insights into Collective Cell Migration

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McMahon, Amy, Supatto, Willy, Fraser, Scott E., Stathopoulos, Angelike·2008·Science
DOI 10.1126/science.1167094PMC PMC2801059
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Abstract

The concerted movement of cells from different germ layers contributes to morphogenesis during early embryonic development. Using an optimized imaging approach and quantitative methods, we analyzed the trajectories of hundreds of ectodermal cells and internalized mesodermal cells within Drosophila embryos over 2 hours during gastrulation. We found a high level of cellular organization, with mesoderm cell movements correlating with some but not all ectoderm movements. During migration, the mesoderm population underwent two ordered waves of cell division and synchronous cell intercalation, and cells at the leading edge stably maintained position. Fibroblast growth factor (FGF) signaling guides mesodermal cell migration; however, we found some directed dorsal migration in an FGF receptor mutant, which suggests that additional signals are involved. Thus, decomposing complex cellular movements can provide detailed insights into collective cell migration.

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  • FGF signaling likely functions at multiple steps during mesoderm migration to control the directed movement of cells.

    “FGF signaling likely functions at multiple steps during the migration to control the directed movement of cells (McMahon et al. 2008;Kadam et al. 2009).”

    From Graded dorsal and differential gene regulation in the Drosophila embryo. (2009) · cites this paper as McMahon et al. 2008

    The supplied passages (indexed under Abstract; page unavailable) show that FGF signaling contributes to mesoderm migration: loss of signaling disrupts furrow collapse and angular spreading. But the paper identifies furrow collapse as FGF’s primary function and says another signal guides directed angular migration, so it does not establish that FGF acts at multiple steps to control that directed movement. (LLM, conf=0.85)

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Authors
McMahon, A., Supatto, W., Fraser, S. E., & Stathopoulos, A.
Affiliations
California Institute of Technology (US)
Corresponding author
  • McMahon, Amy
  • Supatto, Willy
  • Stathopoulos, Angelike
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2008-12-05
Volume
322
Issue
5907
Pages
1546-1550
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.1167094
PMC
PMC2801059
OpenAlex
W2050032205
Cited by
148
References
0
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 6 · 2022: 2 · 2021: 9 · 2020: 8 · 2019: 5 · 2018: 3 · 2017: 7 · 2016: 8 · 2015: 9 · 2014: 12 · 2013: 14 · 2012: 11
FWCI
6.73
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • CaltechAUTHORS (California Institute of Technology) (submittedVersion)
  • HAL (submittedVersion)
Funders
  • National Human Genome Research Institute (P50HG004071)
  • National Institute of General Medical Sciences (R01GM078542)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
Keywords
Drosophila gastrulation, collective cell migration, cell intercalation, FGF signaling, fibroblast growth factor receptor, cell trajectory, embryonic morphogenesis
MeSH terms
Animals, Cell Division, Cell Movement, Drosophila, Ectoderm, Embryo, Nonmammalian, Fibroblast Growth Factors, Mesoderm, Morphogenesis, Mutation, Phenotype, Signal Transduction, Gastrulation

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