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An Oxidative Enzyme Boosting the Enzymatic Conversion of Recalcitrant Polysaccharides
Abstract
Polysaccharide Breakdown: One of the current challenges in the biofuels industry is achieving efficient bioconversion of complex polysaccharides like cellulose and chitin. Recently, chitin-binding proteins (CBPs) have been identified that potentiate chitin hydrolysis. Now, Vaaje-Kolstad et al. (p. 219 ) show that a CBP from the chitinolytic bacterium Serratia marcescens appears to catalyze an oxygenase reaction on the surface of crystallized chitin, leading to chain breakage and generating oxidized ends that can be degraded by chitinases. A structurally similar enzyme, GH61, may play a similar role in the degradation of cellulose.
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Cited by
Payne, C. M., Knott, B. C., Mayes, H. B., Hansson, H., Himmel, M. E., Sandgren, M., Ståhlberg, J., & Beckham, G. T. (2015) · Chemical Reviews · doi:10.1021/cr500351c · Cited by 855
Biological Pretreatment of Lignocellulosic Biomass for Biofuels and Bioproducts: An Overview
Sharma, H. K., Xu, C., & Qin, W. (2019) · Waste and Biomass Valorization · doi:10.1007/s12649-017-0059-y · Cited by 617
Oxidative cleavage of polysaccharides by monocopper enzymes depends on H2O2
Bissaro, B., Røhr, Å. K., Müller, G., Chylenski, P., Skaugen, M., Forsberg, Z., Horn, S. J., Vaaje-Kolstad, G., & Eijsink, V. G. H. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2470 · Cited by 594
Self-citationThe role of the cell wall in plant immunity
Malinovsky, F. G., Fangel, J. U., & Willats, W. G. T. (2014) · Frontiers in Plant Science · doi:10.3389/fpls.2014.00178 · Cited by 555
Visions of sustainability in bioeconomy research
Pfau, S., Hagens, J., Dankbaar, B., & Smits, A. (2014) · Sustainability · doi:10.3390/su6031222 · Cited by 398
“…al. [67] New Biotechnology Resources II Navia et al. [68] Waste [91] Journal of Cleaner Production Processing and Technology I Vaaje-Kolstad et al. [92] Science Processing and Technology II Van Dam et al. [93] Industrial Crops and Products Resources II Vaneeckhaute et al. [94] Water, Air, & Soil…”
Metadata
- Authors
- Vaaje-Kolstad, G., Westereng, B., Horn, S. J., Liu, Z., Zhai, H., Sørlie, M., & Eijsink, V. G. H.
- Venue
- Science
- Published
- 2010-10-08
- Volume
- 330
- Issue
- 6001
- Pages
- 219-222
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2064013442
- Cited by
- 1,341
- References
- 0
- FWCI
- 15.95
- Type
- journal article
- Language
- en
- Open access
- closed
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- Vaaje-Kolstad, G., Westereng, B., Horn, S. J., Liu, Z., Zhai, H., Sørlie, M., & Eijsink, V. G. H. (2010). An Oxidative Enzyme Boosting the Enzymatic Conversion of Recalcitrant Polysaccharides. Science. https://doi.org/10.1126/science.1192231
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