Local Impermeant Anions Establish the Neuronal Chloride Concentration
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Causing Chloride Changes: Because intracellular chloride concentrations largely determine the direction and magnitude of current flow through GABA A channels, the stability of intracellular chloride concentration is important to maintain consistent synaptic inhibition. Glykys et al. (p. 670 ) examined the mechanisms by which chloride gradients in neurons are established, using chloride imaging with transgenically expressed clomeleon dye. Surprisingly, intracellular chloride was not primarily determined by transporters. Instead, subcellular gradients of immobile anions generated inverse chloride gradients.
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Staley and colleagues demonstrated that CSPG digestion changes the transmembrane Cl− gradient, which can alter GABAergic neurotransmission.
“Staley and colleagues demonstrated that CSPGs digestion changes the transmembrane Cl− gradient, which can alter GABAergic neurotransmission20.”
From Perineuronal nets decrease membrane capacitance of peritumoral fast spiking interneurons in a model of epilepsy (2018) · cites this paper as [20]
The extracellular matrix is critical for establishing the local chloride concentration.
“Second, the ECM is critical for establishing the local Cl -concentration (Glykys et al, 2014).”
From Reduced Labeling of Parvalbumin Neurons and Perineuronal Nets in the Dorsolateral Prefrontal Cortex of Subjects with Schizophrenia (2016) · cites this paper as Glykys et al, 2014
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References 40 · Cited by 212
- Authors
- Glykys, J., Dzhala, V., Egawa, K., Balena, T., Saponjian, Y., Kuchibhotla, K. V., Bacskai, B. J., Kahle, K. T., Zeuthen, T., & Staley, K. J.
- Affiliations
- Harvard University (US)
- New York University (US)
- University of Copenhagen (DK)
- Corresponding author
- Glykys, J.
- Dzhala, V.
- Egawa, K.
- Staley, K. J.
- Venue
- Science
- Published
- 2014-02-07
- Volume
- 343
- Issue
- 6171
- Pages
- 670-675
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC4220679
- OpenAlex
- W2052163800
- Cited by
- 212
- References
- 40
- Citations by year
- 2026: 8 · 2025: 8 · 2024: 15 · 2023: 16 · 2022: 18 · 2021: 12 · 2020: 13 · 2019: 19 · 2018: 23 · 2017: 11 · 2016: 21 · 2015: 28 · 2014: 20
- FWCI
- 12.09
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (R01NS074772, NS 40109-06, R01NS040109, R25NS065743)
- National Institute of Biomedical Imaging and Bioengineering (R01EB000768)
- National Center for Research Resources (U41RR019703, S10RR025645)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Keywords
- extracellular matrix glycoprotein, chloride homeostasis
- MeSH terms
- Animals, Brain, Cell Membrane Permeability, Chlorides, Cytoplasm, Glycoproteins, Mice, Transgenic, Neurons, Receptors, GABA-A, Recombinant Fusion Proteins, Signal Transduction, Extracellular Matrix Proteins, Cell Polarity, Chloride Channels, Mice
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