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Immunization of Normal Mouse Spleen Cell Suspensions in vitro
Abstract
Dissociated cells from the spleens of unimmunized mice were cultured with and without various mammalian erythrocytes. Spleen cell suspensions cultured with heterologous red cells developed levels of hemolytic plaque-forming cells only one log 2 less than those seen in vivo. The reaction is specific for the in vitro immunizing erythrocytes. Antibody was demonstrated in the culture fluids.
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Citations
Cited by
Beyond oxidative stress: an immunologist's guide to reactive oxygen species
Nathan, C., & Cunningham-Bussel, A. (2013) · Nature Reviews Immunology · doi:10.1038/nri3423 · Cited by 1,619
Cabeza-Cabrerizo, M., Cardoso, A., Minutti, C. M., Pereira da Costa, M., & Reis e Sousa, C. (2021) · Annual Review of Immunology · doi:10.1146/annurev-immunol-061020-053707 · Cited by 757
A Requirement for Two Cell Types for Antibody Formation in vitro
Mosier, D. E. (1967) · Science · doi:10.1126/science.158.3808.1573 · Cited by 608
Metadata
- Authors
- Mishell, R. I., & Dutton, R. W.
- Venue
- Science
- Published
- 1966-08-26
- Volume
- 153
- Issue
- 3739
- Pages
- 1004-1006
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2037232132
- Cited by
- 229
- References
- 0
- FWCI
- 8.31
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Immunization of Normal Mouse Spleen Cell Suspensions in vitro. https://app.cochat.ai/papers/10.1126/science.153.3739.1004
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- Mishell, R. I., & Dutton, R. W. (1966). Immunization of Normal Mouse Spleen Cell Suspensions in vitro. Science. https://doi.org/10.1126/science.153.3739.1004
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
