CoChat
Sign Up
Cited by 320Pancreatic function and diabetesProtein Structure and DynamicsProtein purification and stability

Porcine Proinsulin: Characterization and Amino Acid Sequence

Chance, Ronald E., Ellis, Robert M., Bromer, William W.·1968·Science
DOI 10.1126/science.161.3837.165
Open this paper in CoChat — free account

Abstract

Proinsulin in nearly homogeneous form has been isolated from a preparation of porcine insulin. A molecular weight close to 9100 was calculated from the amino acid composition and from sedimentation-equilibrium studies. Through the action of trypsin this single-chain protein is transformed to desalanine insulin by cleavage of a polypeptide chain connecting the carboxy-terminus of the B chain to the amino-terminus of the A chain of insulin. The amino acid sequence of this connecting peptide was found to be Arg-Arg-Glu-Ala-Gln-Asn-Pro-Gln-Ala-Gly-Ala-Val-Glu-Leu-Gly-Gly-Gly-Leu-Gly-Gly-Leu-Gln-Ala-Leu-Ala-Leu-Glu-Gly-Pro-Pro-Gln-Lys-Arg.

Integrity signals

Registry

Crossref · 10.1126/science.161.3837.165

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cited by

Sorted by citations

Authors
Chance, R. E., Ellis, R. M., & Bromer, W. W.
Affiliations
Eli Lilly (United States) (US)
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
1968-07-12
Volume
161
Issue
3837
Pages
165-167
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.161.3837.165
OpenAlex
W2093477186
Cited by
320
References
0
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 4 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 1 · 2013: 2 · 2012: 5
FWCI
88.70
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
Keywords
proinsulin, porcine insulin, amino acid sequence, C-peptide, trypsin cleavage, molecular weight, sedimentation equilibrium, polypeptide chains
MeSH terms
Amino Acid Sequence, Animals, Biological Assay, Cellulose, Chromatography, Ion Exchange, Chymotrypsin, Electrophoresis, Disc, Hypoglycemia, Insulin, Molecular Weight, Peptides, Proteins, Seizures, Swine, Trypsin

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review