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Increase of the Catalytic Activity of Phospholipase C-γ1 by Tyrosine Phosphorylation
Abstract
Phospholipase C-γ1 (PLC-γ1), an isozyme of the phosphoinositide-specific phospholipase C family, which occupies a central role in hormonal signal transduction pathways, is an excellent substrate for the epidermal growth factor (EGF) receptor tyrosine kinase. Epidermal growth factor elicits tyrosine phosphorylation of PLC-γ1 and phosphatidylinositol 4,5-bisphosphate hydrolysis in various cell lines. The ability of tyrosine phosphorylation to activate the catalytic activity of PLC-γ1 was tested. Tyrosine phosphorylation in intact cells or in vitro increased the catalytic activity of PLC-γ1. Also, treatment of EGF-activated PLC-γ1 with a tyrosine-specific phosphatase substantially decreased the catalytic activity of PLC-γ1. These results suggest that the EGF-stimulated formation of inositol 1,4,5-trisphosphate and diacylglycerol in intact cells results, at least in part, from catalytic activation of PLC-γ1 through tyrosine phosphorylation.
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Citations
Cited by
Middleton, E., Kandaswami, C., & Theoharides, T. C. (2000) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01472-8 · Cited by 4,882
Epidermal Growth Factor Receptor Cell Proliferation Signaling Pathways
Wee, P., & Wang, Z. (2017) · Cancers · doi:10.3390/cancers9050052 · Cited by 2,027
Signal transduction from the extracellular matrix.
Juliano, R. L., & Haskill, S. (1993) · The Journal of cell biology · doi:10.1083/jcb.120.3.577 · Cited by 1,683
Emergent Properties of Networks of Biological Signaling Pathways
Bhalla, U. S., & Iyengar, R. (1999) · Science · doi:10.1126/science.283.5400.381 · Cited by 1,686
Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation
Balla, T. (2013) · Physiological Reviews · doi:10.1152/physrev.00028.2012 · Cited by 1,638
Metadata
- Authors
- Nishibe, S., Wahl, M. I., Hernandez-Sotomayor, S. M., Tonks, N. K., Rhee, S. G., & Carpenter, G.
- Venue
- Science
- Published
- 1990-11-30
- Volume
- 250
- Issue
- 4985
- Pages
- 1253-1256
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2017100096
- Cited by
- 635
- References
- 0
- FWCI
- 34.58
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Increase of the Catalytic Activity of Phospholipase C-γ1 by Tyrosine Phosphorylation. https://app.cochat.ai/papers/10.1126/science.1700866
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- Nishibe, S., Wahl, M. I., Hernandez-Sotomayor, S. M., Tonks, N. K., Rhee, S. G., & Carpenter, G. (1990). Increase of the Catalytic Activity of Phospholipase C-γ1 by Tyrosine Phosphorylation. Science. https://doi.org/10.1126/science.1700866
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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