Buffer Combinations for Mammalian Cell Culture
Abstract
The growth and metabolism of cultured mammalian cells are markedly affected by the pH variation in ordinary hicarbonate-buffered media (pH 8.0 to 6.9). Those pH swings can be reduced and the pH of the culture can be stabilized as desired in the range pH 6.4 to 8.3 by appropriate combinations of two or three organic buffers, each at 10 to 15 millimolar, in conjunction with phosphate and bicarbonate. The initial alkalinization in sparse cultures is then minimized, and the metabolic acidificatiotn in 24 hours is usually less than 0.4 pH unit except in heavy cultutres.
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Consistent observations of pH instability in standard cell cultures prompted the use of additional exogenous buffers in media formulations to enhance medium buffering capacity.
“Although consistent observations of pH instability in standard cell cultures prompted the use of additional exogenous buffers in media formulations to enhance medium buffering capacity (Eagle, 1971)”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Eagle, 1971
The reported median decline in pH was 0.43 units, but in some particularly extreme cases, cell metabolic activity promoted pH reductions that approached one pH unit and dissolved O2 decreased down to 0.95%.
“The reported median decline in pH was 0.43 units, but in some particularly extreme cases, cell metabolic activity promoted pH reductions that approached one pH unit (Eagle, 1971) and dissolved O2 decreased down to 0.95% (Vallejos et al., 2010).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Eagle, 1971
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References 13 · Cited by 304
- Authors
- Eagle, H.
- Affiliations
- Albert Einstein College of Medicine (US)
- Corresponding author
- Eagle, Harry
- Venue
- Science
- Published
- 1971-10-29
- Volume
- 174
- Issue
- 4008
- Pages
- 500-503
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2064616746
- Cited by
- 304
- References
- 13
- Citations by year
- 2026: 5 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 10 · 2020: 5 · 2019: 6 · 2018: 3 · 2017: 3 · 2016: 3 · 2015: 3 · 2014: 7 · 2013: 7 · 2012: 2
- FWCI
- 11.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Keywords
- mammalian cell culture, pH stabilization, bicarbonate, phosphate, culture medium pH
- MeSH terms
- Animals, Buffers, Cell Transformation, Neoplastic, Cells, Cultured, Fibroblasts, HeLa Cells, Humans, Hydrogen-Ion Concentration, Kidney, Neoplasms, Mice
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