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Branching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy
Abstract
Cobalt chloride can be injected into an identified nerve cell body in an insect ganglion and reacted with ammonium sulfide to stain the soma and its branches with a black precipitate. The stained cell body and its branches throughout the neuropil are visible in both the light and electron microscope. In whole mount preparations, the resolution of neurites within the neuropil is of a quality that permits the comparison of branching patterns between cells and during various functional states.
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Citations
Cited by
Hausen, K. (1982) · Biological Cybernetics · doi:10.1007/bf00335241 · Cited by 363
Monosynaptic connexions between wing stretch receptors and flight motoneurones of the locust
Burrows, M. (1975) · Journal of Experimental Biology · doi:10.1242/jeb.62.1.189 · Cited by 276
Electrical Coupling Between Supernumerary Motor Neurones in the Locust
Siegler, M. V. S. (1982) · Journal of Experimental Biology · doi:10.1242/jeb.101.1.105 · Cited by 51
Schlurmann, M., & Hausen, K. (2007) · Journal of Comparative Neurology · doi:10.1002/cne.21182 · Cited by 11
Metadata
- Authors
- Pitman, R. M., Tweedle, C. D., & Cohen, M. J.
- Venue
- Science
- Published
- 1972-04-28
- Volume
- 176
- Issue
- 4033
- Pages
- 412-414
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1978049645
- Cited by
- 506
- References
- 0
- FWCI
- 18.08
- Type
- journal article
- Language
- en
- Open access
- closed
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- Pitman, R. M., Tweedle, C. D., & Cohen, M. J. (1972). Branching of Central Neurons: Intracellular Cobalt Injection for Light and Electron Microscopy. Science. https://doi.org/10.1126/science.176.4033.412
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
