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Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range
Abstract
Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent. Applications are discussed.
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Research works printed but not linked: 9
- CHEN, R.F., FLUORESCENCE QUANTUM YIELD MEASUREMENTS - VITAMIN B6 COMPOUNDS, SCIENCE 150: 1593 (1965). · doi:10.1126/science.150.3703.1593
- Felix, A. M., Analytical Biochemistry 52: 377 (1973). · doi:10.1016/0003-2697(73)90040-7
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- MCCAMAN, M.W., FLUORIMETRIC METHOD FOR DETERMINATION OF PHENYLALANINE IN SERUM, JOURNAL OF LABORATORY AND CLINICAL MEDICINE 59: 885 (1962).
- SAMEJIMA, K, CONDENSATION OF NINHYDRIN WITH ALDEHYDES AND PRIMARY AMINES TO YIELD HIGHLY FLUORESCENT TERNARY PRODUCTS .1. MECHANISM OF REACTION AND SOME CHARACTERISTICS OF CONDENSATION PRODUCT, ANALYTICAL BIOCHEMISTRY 42: 222 (1971). · doi:10.1016/0003-2697(71)90030-3
- SAMEJIMA, K, CONDENSATION OF NINHYDRIN WITH ALDEHYDES AND PRIMARY AMINES TO YIELD HIGHLY FLUORESCENT TERNARY PRODUCTS .2. APPLICATION TO DETECTION AND ASSAY OF PEPTIDES, AMINO ACIDS, AMINES, AND AMINO SUGARS, ANALYTICAL BIOCHEMISTRY 42: 237 (1971). · doi:10.1016/0003-2697(71)90031-5
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- WEIGELE, M, NOVEL REAGENT FOR FLUOROMETRIC ASSAY OF PRIMARY AMINES, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 94: 5927 (1972). · DOI · Paper page
Cited by
Stewart, J. M. (1976) · Journal of Macromolecular Science: Part A - Chemistry · doi:10.1080/00222337608068099 · Cited by 1,767
von Bergen, M., Friedhoff, P., Biernat, J., Heberle, J., Mandelkow, E.-M., & Mandelkow, E. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.10.5129 · Cited by 1,021
Mack, D., Fischer, W., Krokotsch, A., Leopold, K., Hartmann, R., Egge, H., & Laufs, R. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.1.175-183.1996 · Cited by 885
5 Analysis of the Chemical Composition and Primary Structure of Murein
Schleifer, K. H. (1985) · Methods in Microbiology · doi:10.1016/s0580-9517(08)70474-4 · Cited by 710
Bremer, E. G., Schlessinger, J., & Hakomori, S. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35954-9 · Cited by 706
Metadata
- Authors
- Udenfriend, S., Stein, S., Böhlen, P., Dairman, W., Leimgruber, W., & Weigele, M.
- Venue
- Science
- Published
- 1972-11-24
- Volume
- 178
- Issue
- 4063
- Pages
- 871-872
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2087298783
- Cited by
- 2,649
- References
- 0
- FWCI
- 67.52
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range. https://app.cochat.ai/papers/10.1126/science.178.4063.871
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- Udenfriend, S., Stein, S., Böhlen, P., Dairman, W., Leimgruber, W., & Weigele, M. (1972). Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range. Science. https://doi.org/10.1126/science.178.4063.871
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
