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Regulation of Phospholipase C-γ1 by Profilin and Tyrosine Phosphorylation
Abstract
Epidermal growth factor and platelet-derived growth factor can stimulate the production of the second messenger inositol trisphosphate in responsive cells, but the biochemical pathway for these signaling events has been uncertain because the reactions have not been reconstituted with purified molecules in vitro. A reconstitution is described that requires not only the growth factor, its receptor with tyrosine kinase activity, and the soluble phospholipase C-γ1, but also the small soluble actin-binding protein profilin. Profilin binds to the substrate phosphatidylinositol 4,5-bisphosphate and inhibits its hydrolysis by unphosphorylated phospholipase C-γ1. Phosphorylation of phospholipase C-γ1 by the epidermal growth factor receptor tyrosine kinase overcomes the inhibitory effect of profilin and results in an effective activation of phospholipase C-γ1.
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Cited by
Várnai, P., & Balla, T. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.2.501 · Cited by 859
EGF-induced PIP2 hydrolysis releases and activates cofilin locally in carcinoma cells
van Rheenen, J., Song, X., van Roosmalen, W., Cammer, M., Chen, X., DesMarais, V., Yip, S.-C., Backer, J. M., Eddy, R. J., & Condeelis, J. S. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200706206 · Cited by 244
Kurosaki, T., & Tsukada, S. (2000) · Immunity · doi:10.1016/s1074-7613(00)80153-3 · Cited by 223
Thymosin beta 10 and thymosin beta 4 are both actin monomer sequestering proteins
Yu, F. X., Lin, S. C., Morrison-Bogorad, M., Atkinson, M. A., & Yin, H. L. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54179-x · Cited by 177
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Metadata
- Authors
- Goldschmidt-Clermont, P. J., Kim, J. W., Machesky, L. M., Rhee, S. G., & Pollard, T. D.
- Venue
- Science
- Published
- 1991-03-08
- Volume
- 251
- Issue
- 4998
- Pages
- 1231-1233
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2022129165
- Cited by
- 527
- References
- 0
- FWCI
- 28.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Regulation of Phospholipase C-γ1 by Profilin and Tyrosine Phosphorylation. https://app.cochat.ai/papers/10.1126/science.1848725
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- Goldschmidt-Clermont, P. J., Kim, J. W., Machesky, L. M., Rhee, S. G., & Pollard, T. D. (1991). Regulation of Phospholipase C-γ1 by Profilin and Tyrosine Phosphorylation. Science. https://doi.org/10.1126/science.1848725
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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