The DOI matches its Crossref record.
Network Rigidity and Metabolic Engineering in Metabolite Overproduction
Abstract
In order to enhance the yield and productivity of metabolite production, researchers have focused almost exclusively on enzyme amplification or other modifications of the product pathway. However, overproduction of many metabolites requires significant redirection of flux distributions in the primary metabolism, which may not readily occur following product deregulation because metabolic pathways have evolved to exhibit control architectures that resist flux alterations at branch points. This problem can be addressed through the use of some general concepts of metabolic rigidity, which include a means for identifying and removing rigid branch points within an experimental framework.
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Citations
Cited by
Engineering Cellular Metabolism
Nielsen, J., & Keasling, J. D. (2016) · Cell · doi:10.1016/j.cell.2016.02.004 · Cited by 1,378
Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction
Vallino, J. J., & Stephanopoulos, G. (1993) · Biotechnology and Bioengineering · doi:10.1002/bit.260410606 · Cited by 508
Self-citationOncogenic K‐Ras decouples glucose and glutamine metabolism to support cancer cell growth
Gaglio, D., Metallo, C. M., Gameiro, P. A., Hiller, K., Danna, L. S., Balestrieri, C., Alberghina, L., Stephanopoulos, G., & Chiaradonna, F. (2011) · Molecular Systems Biology · doi:10.1038/msb.2011.56 · Cited by 492
Self-citationMarx, A., de Graaf, A. A., Wiechert, W., Eggeling, L., & Sahm, H. (1996) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19960120)49:2<111::aid-bit1>3.0.co;2-t · Cited by 409
Flux Distributions in Anaerobic, Glucose-Limited Continuous Cultures of Saccharomyces Cerevisiae
Nissen, T. L., Schulze, U., Nielsen, J., & Villadsen, J. (1997) · Microbiology · doi:10.1099/00221287-143-1-203 · Cited by 376
Metadata
- Authors
- Stephanopoulos, G., & Vallino, J. J.
- Venue
- Science
- Published
- 1991-06-21
- Volume
- 252
- Issue
- 5013
- Pages
- 1675-1681
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2049167322
- Cited by
- 594
- References
- 0
- FWCI
- 5.33
- Type
- journal article
- Language
- en
- Open access
- closed
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- Stephanopoulos, G., & Vallino, J. J. (1991). Network Rigidity and Metabolic Engineering in Metabolite Overproduction. Science. https://doi.org/10.1126/science.1904627
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
