The DOI matches its Crossref record.
Interphase and Metaphase Resolution of Different Distances Within the Human Dystrophin Gene
Abstract
Fluorescence in situ hybridization makes possible direct visualization of single sequences not only on chromosomes, but within decondensed interphase nuclei, providing a potentially powerful approach for high-resolution (1 Mb and below) gene mapping and the analysis of nuclear organization. Interphase mapping was able to extend the ability to resolve and order sequences up to two orders of magnitude beyond localization on banded or unbanded chromosomes. Sequences within the human dystrophin gene separated by <100 kb to 1 Mb were visually resolved at interphase by means of standard microscopy. In contrast, distances in the 1-Mb range could not be ordered on the metaphase chromosome length. Analysis of sequences 100 kb to 1 Mb apart indicates a strong correlation between interphase distance and linear DNA distance, which could facilitate a variety of gene-mapping efforts. Results estimate chromatin condensation up to 1 Mb and indicate a comparable condensation for different cell types prepared by different techniques.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Brown, C. J., Hendrich, B. D., Rupert, J. L., Lafrenière, R. G., Xing, Y., Lawrence, J., & Willard, H. F. (1992) · Cell · doi:10.1016/0092-8674(92)90520-m · Cited by 1,310
Self-citationRobinett, C. C., Straight, A., Li, G., Willhelm, C., Sudlow, G., Murray, A., & Belmont, A. S. (1996) · The Journal of cell biology · doi:10.1083/jcb.135.6.1685 · Cited by 720
Macromolecular Domains within the Cell Nucleus
Spector, D. L. (1993) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.09.110193.001405 · Cited by 601
High-resolution mapping of mammalian genes by in situ hybridization to free chromatin.
Heng, H. H., Squire, J., & Tsui, L. C. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.20.9509 · Cited by 516
Ewen, M. E., Xing, Y., Lawrence, J. B., & Livingston, D. M. (1991) · Cell · doi:10.1016/0092-8674(91)90038-z · Cited by 444
Self-citation
Metadata
- Authors
- Lawrence, J. B., Singer, R. H., & McNeil, J. A.
- Venue
- Science
- Published
- 1990-08-24
- Volume
- 249
- Issue
- 4971
- Pages
- 928-932
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2053413122
- Cited by
- 257
- References
- 0
- FWCI
- 11.62
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Interphase and Metaphase Resolution of Different Distances Within the Human Dystrophin Gene. https://app.cochat.ai/papers/10.1126/science.2203143
- The paper
- Lawrence, J. B., Singer, R. H., & McNeil, J. A. (1990). Interphase and Metaphase Resolution of Different Distances Within the Human Dystrophin Gene. Science. https://doi.org/10.1126/science.2203143
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
