The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens
Abstract
Transformed Arabidopsis thaliana plants have been produced by a modified leaf disk transformation-regeneration method. Leaf pieces from sterilely grown plants were precultured for 2 days and inoculated with an Agrobacterium tumefaciens strain containing an avirulent Ti (tumor-inducing) plasmid with a chimeric gene encoding hygromycin resistance. After cocultivation for 2 days, the leaf pieces were placed on a medium that selects for hygromycin resistance. Shoots regenerated within 3 months and were excised, rooted, and transferred to soil. Transformation was confirmed by opine production, hygromycin resistance, and DNA blot hybridization of both primary transformants and progeny. This process for producing transgenic Arabidopsis plants should enhance the usefulness of the species for experimental biology.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes
González, A., Jiménez, A., Vázquez, D., Davies, J. E., & Schindler, D. (1978) · Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis · doi:10.1016/0005-2787(78)90287-3 · Cited by 176
Cited by
Agrobacterium-Mediated Plant Transformation: Biology and Applications
Hwang, H.-H., Yu, M., & Lai, E.-M. (2017) · The Arabidopsis Book · doi:10.1199/tab.0186 · Cited by 312
Meyerowitz, E. M. (1989) · Cell · doi:10.1016/0092-8674(89)90900-8 · Cited by 305
Metadata
- Authors
- Lloyd, A. M., Barnason, A. R., Rogers, S. G., Byrne, M. C., Fraley, R. T., & Horsch, R. B.
- Venue
- Science
- Published
- 1986-10-24
- Volume
- 234
- Issue
- 4775
- Pages
- 464-466
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2053946000
- Cited by
- 218
- References
- 1
- FWCI
- 9.26
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens. https://app.cochat.ai/papers/10.1126/science.234.4775.464
- The paper
- Lloyd, A. M., Barnason, A. R., Rogers, S. G., Byrne, M. C., Fraley, R. T., & Horsch, R. B. (1986). Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens. Science. https://doi.org/10.1126/science.234.4775.464
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
