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Polyhydroxybutyrate, a Biodegradable Thermoplastic, Produced in Transgenic Plants
Abstract
Polyhydroxybutyrate (PHB), a high molecular weight polyester, is accumulated as a storage carbon in many species of bacteria and is a biodegradable thermoplastic. To produce PHB by genetic engineering in plants, genes from the bacterium Alcaligenes eutrophus that encoded the two enzymes required to convert acetoacetyl—coenzyme A to PHB were placed under transcriptional control of the cauliflower mosaic virus 35S promoter and introduced into Arabidopsis thaliana . Transgenic plant lines that contained both genes accumulated PHB as electron-lucent granules in the cytoplasm, nucleus, and vacuole; the size and appearance of these granules were similar to the PHB granules that accumulate in bacteria.
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Citations
Cited by
Comparative organic geochemistries of soils and marine sediments
Hedges, J. I., & Oades, J. M. (1997) · Organic Geochemistry · doi:10.1016/s0146-6380(97)00056-9 · Cited by 744
Spiekermann, P., Rehm, B. H. A., Kalscheuer, R., Baumeister, D., & Steinbüchel, A. (1999) · Archives of Microbiology · doi:10.1007/s002030050681 · Cited by 744
The Chemomechanical Properties of Microbial Polyhydroxyalkanoates
Laycock, B., Halley, P., Pratt, S., Werker, A., & Lant, P. (2013) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2012.06.003 · Cited by 604
Poirier, Y., Nawrath, C., & Somerville, C. (1995) · Nature Biotechnology · doi:10.1038/nbt0295-142 · Cited by 480
Self-citationSteinbüchel, A., & Lütke-Eversloh, T. (2003) · Biochemical Engineering Journal · doi:10.1016/s1369-703x(03)00036-6 · Cited by 470
Metadata
- Authors
- Poirier, Y., Dennis, D. E., Klomparens, K., & Somerville, C.
- Venue
- Science
- Published
- 1992-04-24
- Volume
- 256
- Issue
- 5056
- Pages
- 520-523
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2078182305
- Cited by
- 399
- References
- 0
- FWCI
- 16.92
- Type
- journal article
- Language
- en
- Open access
- closed
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- Poirier, Y., Dennis, D. E., Klomparens, K., & Somerville, C. (1992). Polyhydroxybutyrate, a Biodegradable Thermoplastic, Produced in Transgenic Plants. Science. https://doi.org/10.1126/science.256.5056.520
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