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A Vital Stain for the Golgi Apparatus
Abstract
The Golgi complex, a membranous organelle with important functions in membrane traffic and macromolecular synthesis, has been stained in living cells with a fluorescent sphingolipid. Cells were first incubated with liposomes containing N -[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl sphingosine (C 6 -NBD-ceramide), or with a bovine serum albumin complex of the fluorescent lipid, and then examined by fluorescence microscopy. An intensely fluorescent perinuclear structure was identified as the Golgi apparatus by its colocalization with known Golgi markers in fixed cells. C 6 -NBD-ceramide was used to observe the morphology of the Golgi apparatus in living cells in the presence or absence of monensin or Colcemid, and during mitosis. In all cases, C 6 -NBD-ceramide revealed a Golgi apparatus in the living cell that was identical to that obtained with conventional procedures that require fixation.
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Citations
Cited by
Sondell, M., Lundborg, G., & Kanje, M. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-14-05731.1999 · Cited by 750
“The method for staining of the Golgi apparatus was based on a procedure developed by Lipsky and Pagano (1985).”
MethodA vital role for glycosphingolipid synthesis during development and differentiation
Yamashita, T., Wada, R., Sasaki, T., Deng, C., Bierfreund, U., Sandhoff, K., & Proia, R. L. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.16.9142 · Cited by 505
Golgi Tubule Traffic and the Effects of Brefeldin A Visualized in Living Cells
Sciaky, N., Presley, J., Smith, C., Zaal, K. J. M., Cole, N., Moreira, J. E., Terasaki, M., Siggia, E., & Lippincott-Schwartz, J. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.139.5.1137 · Cited by 434
Chattopadhyay, A. (1990) · Chemistry and Physics of Lipids · doi:10.1016/0009-3084(90)90128-e · Cited by 329
Abnormal processing of Golgi elements and lysosomes in Tangier disease.
Robenek, H., & Schmitz, G. (1991) · Arteriosclerosis and Thrombosis: A Journal of Vascular Biology · doi:10.1161/01.atv.11.4.1007 · Cited by 38
Metadata
- Authors
- Lipsky, N. G., & Pagano, R. E.
- Venue
- Science
- Published
- 1985-05-10
- Volume
- 228
- Issue
- 4700
- Pages
- 745-747
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2034863466
- Cited by
- 346
- References
- 0
- FWCI
- 3.34
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lipsky, N. G., & Pagano, R. E. (1985). A Vital Stain for the Golgi Apparatus. Science. https://doi.org/10.1126/science.2581316
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