The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Complementary DNA Coding Click Beetle Luciferases Can Elicit Bioluminescence of Different Colors
Abstract
Eleven complementary DNA (cDNA) clones were generated from messenger RNA isolated from abdominal light organs of the bioluminescent click beetle, Pyrophorus plagiophthalamus . When expressed in Escherichia coli , these clones can elicit bioluminescence that is readily visible. The clones code for luciferases of four types, distinguished by the colors of bioluminescence they catalyze: green (546 nanometers), yellow-green (560 nanometers), yellow (578 nanometers), and orange (593 nanometers). The amino acid sequences of the different luciferases are 95 to 99 percent identical with each other, but are only 48 percent identical with the sequence of firefly luciferase ( Photinus pyralis ). Because of the different colors, these clones may be useful in experiments in which multiple reporter genes are needed.
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Citations
Cites
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Self-citationAgricultural and biological chemistry
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Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants
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Self-citationCloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.
de Wet, J. R., Wood, K. V., Helinski, D. R., & DeLuca, M. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.23.7870 · Cited by 517
Self-citationIdentification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase.
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Firefly luciferase as a tool in molecular and cell biology
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Cited by
Advances in In Vivo Bioluminescence Imaging of Gene Expression
Contag, C. H., & Bachmann, M. H. (2002) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.4.111901.093336 · Cited by 870
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes
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Where microbiology meets microengineering: design and applications of reporter bacteria
van der Meer, J. R., & Belkin, S. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2392 · Cited by 533
Applications of bioluminescence in biotechnology and beyond
Syed, A. J., & Anderson, J. C. (2021) · Chemical Society Reviews · doi:10.1039/d0cs01492c · Cited by 341
The origin, diversity, and structure function relationships of insect luciferases
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Metadata
- Authors
- Wood, K. V., Lam, Y. A., Seliger, H. H., & McElroy, W. D.
- Venue
- Science
- Published
- 1989-05-12
- Volume
- 244
- Issue
- 4905
- Pages
- 700-702
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2037178607
- Cited by
- 268
- References
- 16
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
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