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Protein Kinase N (PKN) and PKN-Related Protein Rhophilin as Targets of Small GTPase Rho
Abstract
The Rho guanosine 5′-triphosphatase (GTPase) cycles between the active guanosine triphosphate (GTP)-bound form and the inactive guanosine diphosphate-bound form and regulates cell adhesion and cytokinesis, but how it exerts these actions is unknown. The yeast two-hybrid system was used to clone a complementary DNA for a protein (designated Rhophilin) that specifically bound to GTP-Rho. The Rho-binding domain of this protein has 40 percent identity with a putative regulatory domain of a protein kinase, PKN. PKN itself bound to GTP-Rho and was activated by this binding both in vitro and in vivo. This study indicates that a serine-threonine protein kinase is a Rho effector and presents an amino acid sequence motif for binding to GTP-Rho that may be shared by a family of Rho target proteins.
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Citations
Cited by
Rho GTPases and their effector proteins
BISHOP, A. L., & HALL, A. (2000) · Biochemical Journal · doi:10.1042/bj3480241 · Cited by 2,575
Whitmarsh, A. J., & Davis, R. J. (1996) · Journal of Molecular Medicine · doi:10.1007/s001090050063 · Cited by 1,543
Watanabe, N., Madaule, P., Reid, T., Ishizaki, T., Watanabe, G., Kakizuka, A., Saito, Y., Nakao, K., Jockusch, B. M., & Narumiya, S. (1997) · The EMBO Journal · doi:10.1093/emboj/16.11.3044 · Cited by 823
Self-citationActivation of the nuclear factor-kappaB by Rho, CDC42, and Rac-1 proteins
Perona, R., Montaner, S., Saniger, L., Sánchez-Pérez, I., Bravo, R., & Lacal, J. C. (1997) · Genes & Development · doi:10.1101/gad.11.4.463 · Cited by 519
Overexpression of the rhoC gene correlates with progression of ductal adenocarcinoma of the pancreas
Suwa, H., Ohshio, G., Imamura, T., Watanabe, G., Arii, S., Imamura, M., Narumiya, S., Hiai, H., & Fukumoto, M. (1998) · British Journal of Cancer · doi:10.1038/bjc.1998.23 · Cited by 218
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Metadata
- Authors
- Watanabe, G., Saito, Y., Madaule, P., Ishizaki, T., Fujisawa, K., Morii, N., Mukai, H., Ono, Y., Kakizuka, A., & Narumiya, S.
- Venue
- Science
- Published
- 1996-02-02
- Volume
- 271
- Issue
- 5249
- Pages
- 645-648
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2009394026
- Cited by
- 390
- References
- 0
- FWCI
- 22.14
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Protein Kinase N (PKN) and PKN-Related Protein Rhophilin as Targets of Small GTPase Rho. https://app.cochat.ai/papers/10.1126/science.271.5249.645
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- Watanabe, G., Saito, Y., Madaule, P., Ishizaki, T., Fujisawa, K., Morii, N., Mukai, H., Ono, Y., Kakizuka, A., & Narumiya, S. (1996). Protein Kinase N (PKN) and PKN-Related Protein Rhophilin as Targets of Small GTPase Rho. Science. https://doi.org/10.1126/science.271.5249.645
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