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Accurate Processing of a Eukaryotic Precursor Ribosomal RNA by Ribonuclease MRP in Vitro
Abstract
Very few of the enzymes required for eukaryotic precursor ribosomal RNA (pre-rRNA) processing have been identified. Ribonuclease (RNase) MRP was characterized as a nuclease that cleaves mitochondrial replication primers, but it is predominantly nucleolar. Previous genetic evidence revealed that this ribonucleoprotein is required, directly or indirectly, for cleavage of the yeast pre-rRNA in vivo at site A 3 . Here, an in vitro processing system that accurately reproduces this cleavage is described. Biochemical purification and the use of extracts depleted of the MRP RNA demonstrate that endonucleolytic cleavage of the pre-rRNA is directly mediated by RNase MRP. This establishes a role for RNase MRP in the nucleolus.
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Citations
Cited by
The mitochondrial genome: structure, transcription, translation and replication
Taanman, J.-W. (1999) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(98)00161-3 · Cited by 1,635
Ribosome Biogenesis in the Yeast Saccharomyces cerevisiae
Woolford, J. L., & Baserga, S. J. (2013) · Genetics · doi:10.1534/genetics.113.153197 · Cited by 757
The post-transcriptional steps of eukaryotic ribosome biogenesis
Henras, A. K., Soudet, J., Gérus, M., Lebaron, S., Caizergues-Ferrer, M., Mougin, A., & Henry, Y. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-008-8027-0 · Cited by 521
Balakin, A. G., Smith, L., & Fournier, M. J. (1996) · Cell · doi:10.1016/s0092-8674(00)80156-7 · Cited by 468
RIBONUCLEASE P: Unity and Diversity in a tRNA Processing Ribozyme
Frank, D. N., & Pace, N. R. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.153 · Cited by 465
Metadata
- Authors
- Lygerou, Z., Allmang, C., Tollervey, D., & Séraphin, B.
- Venue
- Science
- Published
- 1996-04-12
- Volume
- 272
- Issue
- 5259
- Pages
- 268-270
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2080055531
- Cited by
- 269
- References
- 0
- FWCI
- 7.79
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lygerou, Z., Allmang, C., Tollervey, D., & Séraphin, B. (1996). Accurate Processing of a Eukaryotic Precursor Ribosomal RNA by Ribonuclease MRP in Vitro. Science. https://doi.org/10.1126/science.272.5259.268
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