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Mapping of Catalytic Residues in the RNA Polymerase Active Center
Abstract
When the Mg 2+ ion in the catalytic center of Escherichia coli RNA polymerase (RNAP) is replaced with Fe 2+ , hydroxyl radicals are generated. In the promoter complex, such radicals cleave template DNA near the transcription start site, whereas the β′ subunit is cleaved at a conserved motif NADFDGD (Asn-Ala-Asp-Phe-Asp-Gly-Asp). Substitution of the three aspartate residues with alanine creates a dominant lethal mutation. The mutant RNAP is catalytically inactive but can bind promoters and form an open complex. The mutant fails to support Fe 2+ -induced cleavage of DNA or protein. Thus, the NADFDGD motif is involved in chelation of the active center Mg 2+ .
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Citations
Cited by
Crystal Structure of Thermus aquaticus Core RNA Polymerase at 3.3 Å Resolution
Zhang, G., Campbell, E. A., Minakhin, L., Richter, C., Severinov, K., & Darst, S. A. (1999) · Cell · doi:10.1016/s0092-8674(00)81515-9 · Cited by 813
Nudler, E., Mustaev, A., Goldfarb, A., & Lukhtanov, E. (1997) · Cell · doi:10.1016/s0092-8674(00)80180-4 · Cited by 440
Self-citationMetal Activation of Enzymes in Nucleic Acid Biochemistry
Cowan, J. A. (1998) · Chemical Reviews · doi:10.1021/cr960436q · Cited by 406
A Signal Transduction System that Responds to Extracellular Iron
Wösten, M. M. S. M., Kox, L. F. F., Chamnongpol, S., Soncini, F. C., & Groisman, E. A. (2000) · Cell · doi:10.1016/s0092-8674(00)00092-1 · Cited by 377
Structural basis for substrate loading in bacterial RNA polymerase
Vassylyev, D. G., Vassylyeva, M. N., Zhang, J., Palangat, M., Artsimovitch, I., & Landick, R. (2007) · Nature · doi:10.1038/nature05931 · Cited by 365
Metadata
- Authors
- Zaychikov, E., Martin, E., Denissova, L., Kozlov, M., Markovtsov, V., Kashlev, M., Heumann, H., Nikiforov, V., Goldfarb, A., & Mustaev, A.
- Venue
- Science
- Published
- 1996-07-05
- Volume
- 273
- Issue
- 5271
- Pages
- 107-109
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2046128235
- Cited by
- 159
- References
- 0
- FWCI
- 8.22
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zaychikov, E., Martin, E., Denissova, L., Kozlov, M., Markovtsov, V., Kashlev, M., Heumann, H., Nikiforov, V., Goldfarb, A., & Mustaev, A. (1996). Mapping of Catalytic Residues in the RNA Polymerase Active Center. Science. https://doi.org/10.1126/science.273.5271.107
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
