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Purification and Molecular Cloning of Plx1, a Cdc25-Regulatory Kinase from Xenopus Egg Extracts
Abstract
Cdc2, the cyclin-dependent kinase that controls mitosis, is negatively regulated by phosphorylation on its threonine-14 and tyrosine-15 residues. Cdc25, the phosphatase that dephosphorylates both of these residues, undergoes activation and phosphorylation by multiple kinases at mitosis. Plx1, a kinase that associates with and phosphorylates the amino-terminal domain of Cdc25, was purified extensively from Xenopus egg extracts. Cloning of its complementary DNA revealed that Plx1 is related to the Polo family of protein kinases. Recombinant Plx1 phosphorylated Cdc25 and stimulated its activity in a purified system. Cdc25 phosphorylated by Plx1 reacted strongly with MPM-2, a monoclonal antibody to mitotic phosphoproteins. These studies indicate that Plx1 may participate in control of mitotic progression.
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Citations
Cited by
Polo on the Rise—from Mitotic Entry to Cytokinesis with Plk1
Petronczki, M., Lénárt, P., & Peters, J.-M. (2008) · Developmental Cell · doi:10.1016/j.devcel.2008.04.014 · Cited by 485
M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCF β-TrCP
Watanabe, N., Arai, H., Nishihara, Y., Taniguchi, M., Watanabe, N., Hunter, T., & Osada, H. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0307700101 · Cited by 472
Mitotic Regulation of the APC Activator Proteins CDC20 and CDH1
Kramer, E. R., Scheuringer, N., Podtelejnikov, A. V., Mann, M., & Peters, J.-M. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.5.1555 · Cited by 455
The Aurora kinases: Role in cell transformation and tumorigenesis
Katayama, H., Brinkley, W. R., & Sen, S. (2003) · Cancer and Metastasis Reviews · doi:10.1023/a:1023789416385 · Cited by 398
Polo-like kinases (Plks) and cancer
Takai, N., Hamanaka, R., Yoshimatsu, J., & Miyakawa, I. (2005) · Oncogene · doi:10.1038/sj.onc.1208272 · Cited by 386
Metadata
- Authors
- Kumagai, A., & Dunphy, W. G.
- Venue
- Science
- Published
- 1996-09-06
- Volume
- 273
- Issue
- 5280
- Pages
- 1377-1380
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1974559056
- Cited by
- 507
- References
- 0
- FWCI
- 14.14
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kumagai, A., & Dunphy, W. G. (1996). Purification and Molecular Cloning of Plx1, a Cdc25-Regulatory Kinase from Xenopus Egg Extracts. Science. https://doi.org/10.1126/science.273.5280.1377
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