The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Structure of Nitric Oxide Synthase Oxygenase Dimer with Pterin and Substrate
Abstract
Crystal structures of the murine cytokine-inducible nitric oxide synthase oxygenase dimer with active-center water molecules, the substrate l -arginine ( l -Arg), or product analog thiocitrulline reveal how dimerization, cofactor tetrahydrobiopterin, and l -Arg binding complete the catalytic center for synthesis of the essential biological signal and cytotoxin nitric oxide. Pterin binding refolds the central interface region, recruits new structural elements, creates a 30 angstrom deep active-center channel, and causes a 35° helical tilt to expose a heme edge and the adjacent residue tryptophan-366 for likely reductase domain interactions and caveolin inhibition. Heme propionate interactions with pterin and l -Arg suggest that pterin has electronic influences on heme-bound oxygen. l -Arginine binds to glutamic acid–371 and stacks with heme in an otherwise hydrophobic pocket to aid activation of heme-bound oxygen by direct proton donation and thereby differentiate the two chemical steps of nitric oxide synthesis.
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Nitric oxide synthases: regulation and function
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Heme Enzyme Structure and Function
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Self-citation
Metadata
- Authors
- Crane, B. R., Arvai, A. S., Ghosh, D. K., Wu, C., Getzoff, E. D., Stuehr, D. J., & Tainer, J. A.
- Venue
- Science
- Published
- 1998-03-27
- Volume
- 279
- Issue
- 5359
- Pages
- 2121-2126
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2045639174
- Cited by
- 668
- References
- 35
- FWCI
- 49.93
- Type
- journal article
- Language
- en
- Open access
- closed
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- Crane, B. R., Arvai, A. S., Ghosh, D. K., Wu, C., Getzoff, E. D., Stuehr, D. J., & Tainer, J. A. (1998). Structure of Nitric Oxide Synthase Oxygenase Dimer with Pterin and Substrate. Science. https://doi.org/10.1126/science.279.5359.2121
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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