The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Controlling Gene Expression in Living Cells Through Small Molecule-RNA Interactions
Abstract
Short RNA aptamers that specifically bind to a wide variety of ligands in vitro can be isolated from randomized pools of RNA. Here it is shown that small molecule aptamers also bound their ligand in vivo, enabling development of a method for controlling gene expression in living cells. Insertion of a small molecule aptamer into the 5′ untranslated region of a messenger RNA allowed its translation to be repressible by ligand addition in vitro as well as in mammalian cells. The ability of small molecules to control expression of specific genes could facilitate studies in many areas of biology and medicine.
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Citations
Cites
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In vitro selection of RNA molecules that bind specific ligands
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Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Gossen, M., & Bujard, H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5547 · Cited by 4,943
Controlling Signal Transduction with Synthetic Ligands
Spencer, D. M., Wandless, T. J., Schreiber, S. L., & Crabtree, G. R. (1993) · Science · doi:10.1126/science.7694365 · Cited by 843
Starting at the Beginning, Middle, and End: Translation Initiation in Eukaryotes
Sachs, A. B., Sarnow, P., & Hentze, M. W. (1997) · Cell · doi:10.1016/s0092-8674(00)80268-8 · Cited by 679
Regulation of gene expression by small molecules
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Regulation of Translation in Eukaryotic Systems
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Cited by
Adaptive Recognition by Nucleic Acid Aptamers
Hermann, T., & Patel, D. J. (2000) · Science · doi:10.1126/science.287.5454.820 · Cited by 1,650
Isaacs, F. J., Dwyer, D. J., & Collins, J. J. (2006) · Nature Biotechnology · doi:10.1038/nbt1208 · Cited by 373
Liu, S., Yu, J.-M., Gan, Y.-C., Qiu, X.-Z., Gao, Z.-C., Wang, H., Chen, S.-X., Xiong, Y., Liu, G.-H., Lin, S.-E., et al. (2023) · Military Medical Research · doi:10.1186/s40779-023-00448-w · Cited by 357
Control of mammalian translation by mRNA structure near caps
Babendure, J. R., Babendure, J. L., Ding, J.-H., & Tsien, R. Y. (2006) · RNA · doi:10.1261/rna.2309906 · Cited by 339
Engineering an allosteric transcription factor to respond to new ligands
Taylor, N. D., Garruss, A. S., Moretti, R., Chan, S., Arbing, M. A., Cascio, D., Rogers, J. K., Isaacs, F. J., Kosuri, S., Baker, D., et al. (2016) · Nature Methods · doi:10.1038/nmeth.3696 · Cited by 328
Metadata
- Authors
- Werstuck, G., & Green, M. R.
- Venue
- Science
- Published
- 1998-10-09
- Volume
- 282
- Issue
- 5387
- Pages
- 296-298
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1989297603
- Cited by
- 422
- References
- 13
- FWCI
- 5.40
- Type
- journal article
- Language
- en
- Open access
- closed
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- Werstuck, G., & Green, M. R. (1998). Controlling Gene Expression in Living Cells Through Small Molecule-RNA Interactions. Science. https://doi.org/10.1126/science.282.5387.296
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
