Cited by 363Neutrophil, Myeloperoxidase and Oxidative MechanismsAntimicrobial Peptides and ActivitiesProtease and Inhibitor Mechanisms

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Degradation of Outer Membrane Protein A in Escherichia coli Killing by Neutrophil Elastase

Belaaouaj, Abderr azzaq, Kim, Kwang Sik, Shapiro, Steven D.·2000·Science

DOI 10.1126/science.289.5482.1185

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Abstract

In determining the mechanism of neutrophil elastase (NE)-mediated killing of Escherichia coli, we found that NE degraded outer membrane protein A (OmpA), localized on the surface of Gram-negative bacteria. NE killed wild-type, but not OmpA-deficient, E. coli. Also, whereas NE-deficient mice had impaired survival in response to E. coli sepsis, as compared to wild-type mice, the presence or absence of NE had no influence on survival in response to sepsis that had been induced with OmpA-deficient E. coli. These findings define a mechanism of nonoxidative bacterial killing by NE and point to OmpA as a bacterial target in host defense.

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Mice lacking neutrophil elastase reveal impaired host defense against gram negative bacterial sepsis
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Authors: Belaaouaj, A. A., Kim, K. S., & Shapiro, S. D.
Affiliations: Washington University in St. Louis (US)
Children's Hospital Los Angeles (US)
Corresponding author: Belaaouaj, Abderr azzaq
ORCID: Kim, Kwang Sik
Shapiro, Steven D.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2000-08-18
Volume: 289
Issue: 5482
Pages: 1185-1187
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.289.5482.1185
OpenAlex: W2027297714
Cited by: 363
References: 15
Citations by year: 2026: 7 · 2025: 10 · 2024: 9 · 2023: 10 · 2022: 11 · 2021: 15 · 2020: 15 · 2019: 20 · 2018: 24 · 2017: 12 · 2016: 13 · 2015: 16 · 2014: 15 · 2013: 17 · 2012: 15
FWCI: 4.95
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
Keywords: neutrophil elastase, OmpA, Escherichia coli, neutrophil killing, Gram-negative bacteria, sepsis, host defense, bacterial surface proteins
MeSH terms: Animals, Bacterial Outer Membrane Proteins, Blood Bactericidal Activity, Cell Membrane, Escherichia coli, Escherichia coli Infections, Humans, Microscopy, Electron, Mutation, Neutrophils, Phagosomes, Proteins, Colony Count, Microbial, Serine Proteinase Inhibitors, Leukocyte Elastase, Mice, Proteinase Inhibitory Proteins, Secretory

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Degradation of Outer Membrane Protein A in Escherichia coli Killing by Neutrophil Elastase

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