The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
The Productive Conformation of Arachidonic Acid Bound to Prostaglandin Synthase
Abstract
Prostaglandin H synthase-1 and -2 (PGHS-1 and -2) catalyze the committed step in prostaglandin synthesis and are targets for nonsteroidal anti-inflammatory drugs (NSAIDs) like aspirin. We have determined the structure of PGHS-1 at 3 angstrom resolution with arachidonic acid (AA) bound in a chemically productive conformation. The fatty acid adopts an extended L-shaped conformation that positions the 13pro S hydrogen of AA for abstraction by tyrosine-385, the likely radical donor. A space also exists for oxygen addition on the antarafacial surface of the carbon in the 11-position (C-11). While this conformation allows endoperoxide formation between C-11 and C-9, it also implies that a subsequent conformational rearrangement must occur to allow formation of the C-8/C-12 bond and to position C-15 for attack by a second molecule of oxygen.
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PROCHECK: a program to check the stereochemical quality of protein structures
Laskowski, R. A., MacArthur, M. W., Moss, D. S., & Thornton, J. M. (1993) · Journal of Applied Crystallography · doi:10.1107/s0021889892009944 · Cited by 24,581
Cyclooxygenases: Structural, cellular, and molecular biology
Smith, W. L., DeWitt, D. L., & Garavito, R. M. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.145 · Cited by 2,925
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Smith, W. L., Urade, Y., & Jakobsson, P.-J. (2011) · Chemical Reviews · doi:10.1021/cr2002992 · Cited by 503
Self-citationWada, M., DeLong, C. J., Hong, Y. H., Rieke, C. J., Song, I., Sidhu, R. S., Yuan, C., Warnock, M., Schmaier, A. H., Yokoyama, C., et al. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m703169200 · Cited by 400
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Metadata
- Authors
- Malkowski, M. G., Ginell, S. L., Smith, W. L., & Garavito, R. M.
- Venue
- Science
- Published
- 2000-09-15
- Volume
- 289
- Issue
- 5486
- Pages
- 1933-1937
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2010967782
- Cited by
- 308
- References
- 3
- FWCI
- 13.00
- Type
- journal article
- Language
- en
- Open access
- closed
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