Affinity Selection from Biological Libraries
Abstract
Phage display is a simple yet powerful technology that is used to rapidly characterize protein-protein interactions from amongst billions of candidates. This widely practiced technique is used to map antibody epitopes, create vaccines and to engineer peptides, antibodies and other proteins as both diagnostic tools and as human therapeutics. We overview the history of phage display and several recent applications.
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Phage display technology has been widely applied in various studies, including protein-protein interactions, antigen epitope screening, and peptide screening.
“Phage display technology has been widely applied in various studies, including protein-protein interactions (PPIs), antigen epitope screening,42 peptide screening and so on.43”
From Identification of heptapeptides targeting a lethal bacterial strain in septic mice through an integrative approach (2022) · cites this paper as [43]
Cited by
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Zhang, X., Li, S., Luo, H., He, S., Yang, H., Li, L., Tian, T., Han, Q., Ye, J., Huang, C., et al. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01035-6 · Cited by 14 · see citing works
- Authors
- Smothers, J. F., Henikoff, S., & Carter, P.
- Affiliations
- Amgen (United States) (US)
- Howard Hughes Medical Institute (US)
- Venue
- Science
- Published
- 2002-10-18
- Volume
- 298
- Issue
- 5593
- Pages
- 621-622
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1669150895
- Cited by
- 67
- References
- 0
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2020: 2 · 2019: 1 · 2017: 3 · 2016: 1 · 2015: 1 · 2014: 4 · 2013: 3 · 2012: 4
- FWCI
- 5.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chemical Synthesis and Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Keywords
- phage display, affinity selection, protein-protein interaction, epitope mapping, vaccine development, peptide engineering, antibody engineering, protein engineering
- MeSH terms
- Animals, Antibodies, Antibody Affinity, Bacteriophages, Humans, Peptides, Protein Binding, Proteins, Recombinant Fusion Proteins, Semiconductors, Epitope Mapping, Peptide Library, Databases, Protein, Nanotechnology
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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