The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Induction of Membrane Ruffling and Fluid-Phase Pinocytosis in Quiescent Fibroblasts by ras Proteins
Abstract
Expression of the ras oncogene is thought to be one of the contributing events in the initiation of certain types of human cancer. To determine the cellular activities that are directly triggered by ras proteins, the early consequences of microinjection of the human H- ras proteins into quiescent rat embryo fibroblasts were investigated. Within 30 minutes to 1 hour after injection, cells show a marked increase in surface ruffles and fluid-phase pinocytosis. The rapid enhancement of membrane ruffling and pinocytosis is induced by both the proto-oncogenic and the oncogenic forms of the H- ras protein. The effects produced by the oncogenic protein persist for more than 15 hours after injection, whereas the effects of the proto-oncogenic protein are short-lived, being restricted to a 3-hour interval after injection. The stimulatory effect of the ras oncogene protein on ruffling and pinocytosis is dependent on the amount of injected protein and is accompanied by an apparent stimulation of phospholipase A 2 activity. These rapid changes in cell membrane activities induced by ras proteins may represent primary events in the mechanism of action of ras proteins.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The ability to stimulate macropinocytosis, a regulated form of endocytosis, is a distinctive feature of KRAS∗ activation,13 and PDAC cells harboring KRAS∗ rely on increased levels of macropinocytosis for nutrient salvaging to sustain uncontrolled cell growth.14”
From Therapeutic targeting of syndecan-1 axis overcomes acquired resistance to KRAS-targeted therapy in gastrointestinal cancers (2025) · cites this paper as [13]
In the citing paper
“The autophagy-MHC-I relationship provides mechanistic insight to functional genomics studies in mice, which have also linked autophagy and immune evasion in PDAC228.”
From Pancreatic cancer: Advances and challenges (2023) · cites this paper as [228]
Citations
Cites
Inositol trisphosphate and diacylglycerol as second messengers
Berridge, M. J. (1984) · Biochemical Journal · doi:10.1042/bj2200345 · Cited by 3,506
G proteins and dual control of adenylate cyclase
Gilman, A. G. (1984) · Cell · doi:10.1016/0092-8674(84)90336-2 · Cited by 1,772
Mechanism of activation of a human oncogene
Tabin, C. J., Bradley, S. M., Bargmann, C. I., Weinberg, R. A., Papageorge, A. G., Scolnick, E. M., Dhar, R., Lowy, D. R., & Chang, E. H. (1982) · Nature · doi:10.1038/300143a0 · Cited by 1,393
Reddy, E. P., Reynolds, R. K., Santos, E., & Barbacid, M. (1982) · Nature · doi:10.1038/300149a0 · Cited by 1,391
Intracellular phospholipases A
van den BOSCH, H. (1980) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(80)90574-x · Cited by 982
Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene
Parada, L. F., Tabin, C. J., Shih, C., & Weinberg, R. A. (1982) · Nature · doi:10.1038/297474a0 · Cited by 947
Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells
Mulcahy, L. S., Smith, M. R., & Stacey, D. W. (1985) · Nature · doi:10.1038/313241a0 · Cited by 851
Expression of Cellular Oncogenes in Human Malignancies
Slamon, D. J., deKernion, J. B., Verma, I. M., & Cline, M. J. (1984) · Science · doi:10.1126/science.6538699 · Cited by 810
Zarbl, H., Sukumar, S. V., Arthur, A. V., Martı́n-Zanca, D., & Barbacid, M. (1985) · Nature · doi:10.1038/315382a0 · Cited by 807
Der, C. J., Krontiris, T. G., & Cooper, G. M. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.11.3637 · Cited by 793
Research works printed but not linked: 2
- ALLISON, A.C., SYMPOSIA OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY 28: 419 (1974).
- McClure, D. B., Cold Spring Harbor Conferences on Cell Proliferation 9: 345 (1982).
Cited by
Sorted by citations
The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
Ridley, A. J., Paterson, H. F., Johnston, C. L., Diekmann, D., & Hall, A. (1992) · Cell · doi:10.1016/0092-8674(92)90164-8 · Cited by 3,616
Takai, Y., Sasaki, T., & Matozaki, T. (2001) · Physiological Reviews · doi:10.1152/physrev.2001.81.1.153 · Cited by 2,511
Role of platelet-derived growth factors in physiology and medicinePreprint
Andræ, J., Gallini, R., & Betsholtz, C. (2008) · Genes & Development · doi:10.1101/gad.1653708 · Cited by 2,534
From Krebs to clinic: glutamine metabolism to cancer therapy
Altman, B. J., Stine, Z. E., & Dang, C. V. (2016) · Nature Reviews Cancer · doi:10.1038/nrc.2016.71 · Cited by 2,119
Drugging the undruggable RAS: Mission Possible?
Cox, A. D., Fesik, S. W., Kimmelman, A. C., Luo, J., & Der, C. J. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4389 · Cited by 1,997
Metadata
- Authors
- Bar-Sagi, D., & Feramisco, J. R.
- Affiliations
- Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724
- Cold Spring Harbor Laboratory (US)
- Venue
- Science
- Published
- 1986-09-05
- Volume
- 233
- Issue
- 4768
- Pages
- 1061-1068
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1969560454
- Cited by
- 734
- References
- 60
- Citations by year
- 2026: 6 · 2025: 15 · 2024: 17 · 2023: 23 · 2022: 17 · 2021: 21 · 2020: 17 · 2019: 25 · 2018: 23 · 2017: 19 · 2016: 14 · 2015: 25 · 2014: 14 · 2013: 11 · 2012: 5
- FWCI
- 14.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA39811, CA07896)
- National Institute of General Medical Sciences (GM 28277)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- H-Ras, oncogenic Ras, ruffles, fluid-phase pinocytosis, quiescent fibroblasts, phospholipase A2, cell membrane dynamics, microinjection, tumor initiation
- MeSH terms
- Animals, Cell Cycle, Cell Membrane, Cells, Cultured, Culture Media, DNA, Humans, Microinjections, Oncogene Proteins, Viral, Phospholipases A, Phospholipids, Pinocytosis, Time Factors, GTP-Binding Proteins, Rats, Phospholipases A2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
