The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Molecular Cloning of the Complementary DNA for Human Tumor Necrosis Factor
Abstract
Tumor necrosis factor (TNF) is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF was purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and showed cytotoxic and cytostatic activities against various human tumor cell lines. The amino acid sequence was determined for the amino terminal end of the purified protein, and oligodeoxyribonucleotide probes were synthesized on the basis of this sequence. Complementary DNA (cDNA) encoding human TNF was cloned from induced HL-60 messenger RNA and was confirmed by hybrid-selection assay, direct expression in COS-7 cells, and nucleotide sequence analysis. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. Expression of high levels of human TNF in Escherichia coli was accomplished under control of the bacteriophage λP L promoter and gene N ribosome binding site.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
An endotoxin-induced serum factor that causes necrosis of tumors
Carswell, E. A., Old, L. J., Kassel, R. L., Green, S., Fiore, N., & Williamson, B. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.9.3666 · Cited by 4,532
Berger, S. L., & Birkenmeier, C. S. (1979) · Biochemistry · doi:10.1021/bi00590a018 · Cited by 443
Cited by
Secretory products of macrophages.
Nathan, C. F. (1987) · Journal of Clinical Investigation · doi:10.1172/jci112815 · Cited by 2,615
Cachectin and tumour necrosis factor as two sides of the same biological coin
Beutler, B., & Cerami, A. (1986) · Nature · doi:10.1038/320584a0 · Cited by 1,647
Sherry, B., & Cerami, A. (1988) · The Journal of cell biology · doi:10.1083/jcb.107.4.1269 · Cited by 432
Torti, S. V., Kwak, E. L., Miller, S. C., Miller, L. L., Ringold, G. M., Myambo, K. B., Young, A. P., & Torti, F. M. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)37801-3 · Cited by 295
Kettelhut, I. C., Fiers, W., & Goldberg, A. L. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.12.4273 · Cited by 286
Metadata
- Authors
- Wang, A. M., Creasey, A. A., Ladner, M. B., Lin, L. S., Strickler, J., Van Arsdell, J. N., Yamamoto, R., & Mark, D. F.
- Venue
- Science
- Published
- 1985-04-12
- Volume
- 228
- Issue
- 4696
- Pages
- 149-154
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1982213879
- Cited by
- 622
- References
- 3
- FWCI
- 36.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wang, A. M., Creasey, A. A., Ladner, M. B., Lin, L. S., Strickler, J., Van Arsdell, J. N., Yamamoto, R., & Mark, D. F. (1985). Molecular Cloning of the Complementary DNA for Human Tumor Necrosis Factor. Science. https://doi.org/10.1126/science.3856324
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