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The "Spliceosome": Yeast Pre-Messenger RNA Associates with a 40 S Complex in a Splicing-Dependent Reaction
Abstract
The in vitro splicing reactions of pre-messenger RNA (pre-mRNA) in a yeast extract were analyzed by glycerol gradient centrifugation. Labeled pre-mRNA appears in a 40 S peak only if the pre-mRNA undergoes the first of the two partial splicing reactions. RNA analysis after extraction of glycerol gradient fractions shows that lariat-form intermediates, molecules that occur only in mRNA splicing, are found almost exclusively in this 40 S complex. Another reaction intermediate, cut 5′ exon RNA, can also be found concentrated in this complex. The complex is stable even in 400 m M KCl, although at this salt concentration, it sediments at 35 S and is clearly distinguishable from 40 S ribosomal subunits. This complex, termed a "spliceosome," is thought to contain components necessary for mRNA splicing; its existence can explain how separated exons on pre-mRNA are brought into contact.
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Citations
Cited by
RNA Splicing by the Spliceosome
Wilkinson, M. E., Charenton, C., & Nagai, K. (2020) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-091719-064225 · Cited by 718
Sharp, P. (1994) · Cell · doi:10.1016/0092-8674(94)90130-9 · Cited by 567
Splice-switching antisense oligonucleotides as therapeutic drugs
Havens, M. A., & Hastings, M. L. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw533 · Cited by 538
Krainer, A. R., & Maniatis, T. (1985) · Cell · doi:10.1016/0092-8674(85)90269-7 · Cited by 419
Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm
Legrain, P., & Rosbash, M. (1989) · Cell · doi:10.1016/0092-8674(89)90127-x · Cited by 406
Metadata
- Authors
- Brody, E., & Abelson, J.
- Venue
- Science
- Published
- 1985-05-24
- Volume
- 228
- Issue
- 4702
- Pages
- 963-967
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2008422577
- Cited by
- 401
- References
- 0
- FWCI
- 21.01
- Type
- journal article
- Language
- en
- Open access
- closed
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- Brody, E., & Abelson, J. (1985). The "Spliceosome": Yeast Pre-Messenger RNA Associates with a 40 S Complex in a Splicing-Dependent Reaction. Science. https://doi.org/10.1126/science.3890181
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