The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The Intervening Sequence RNA of Tetrahymena Is an Enzyme
Abstract
A shortened form of the self-splicing ribosomal RNA (rRNA) intervening sequence of Tetrahymena thermophila acts as an enzyme in vitro. The enzyme catalyzes the cleavage and rejoining of oligonucleotide substrates in a sequence-dependent manner with K m = 42 μ M and k cat = 2 min-1. The reaction mechanism resembles that of rRNA precursor self-splicing. With pentacytidylic acid as the substrate, successive cleavage and rejoining reactions lead to the synthesis of polycytidylic acid. Thus, the RNA molecule can act as an RNA polymerase, differing from the protein enzyme in that it uses an internal rather than an external template. At p H 9, the same RNA enzyme has activity as a sequence-specific ribonuclease.
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Citations
Cites
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme
Guerrier-Takada, C., Gardiner, K., Marsh, T., Pace, N., & Altman, S. (1983) · Cell · doi:10.1016/0092-8674(83)90117-4 · Cited by 2,684
Cited by
Gilbert, W. (1986) · Nature · doi:10.1038/319618a0 · Cited by 2,721
Westheimer, F. H. (1987) · Science · doi:10.1126/science.2434996 · Cited by 1,711
A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis
Greider, C. W., & Blackburn, E. H. (1989) · Nature · doi:10.1038/337331a0 · Cited by 1,643
HITS-CLIP yields genome-wide insights into brain alternative RNA processing
Licatalosi, D. D., Mele, A., Fak, J. J., Ule, J., Kayikci, M., Chi, S. W., Clark, T. A., Schweitzer, A. C., Blume, J. E., Wang, X., et al. (2008) · Nature · doi:10.1038/nature07488 · Cited by 1,352
“Left: qRT–PCR analysis of cells transfected with WT or mutant (three YACY mutations) poly(A) reporter constructs co-transfected with a control (2) or Nova2 expressing (1) plasmid, or WT versus Nova2 KO neocortex (*P , 0.”
In Vitro Selection of Functional Nucleic Acids
Wilson, D. S., & Szostak, J. W. (1999) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.68.1.611 · Cited by 1,206
Metadata
- Authors
- Zaug, A. J., & Cech, T. R.
- Venue
- Science
- Published
- 1986-01-31
- Volume
- 231
- Issue
- 4737
- Pages
- 470-475
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2154989771
- Cited by
- 505
- References
- 1
- FWCI
- 14.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zaug, A. J., & Cech, T. R. (1986). The Intervening Sequence RNA of Tetrahymena Is an Enzyme. Science. https://doi.org/10.1126/science.3941911
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
