The DOI matches its Crossref record.
Expression of a Bacterial Gene in Mammalian Cells
Abstract
Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.
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Cited by
Introns increase transcriptional efficiency in transgenic mice.
Brinster, R. L., Allen, J. M., Behringer, R. R., Gelinas, R. E., & Palmiter, R. D. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.3.836 · Cited by 722
Albritton, L. M., Tseng, L., Scadden, D., & Cunningham, J. M. (1989) · Cell · doi:10.1016/0092-8674(89)90134-7 · Cited by 688
Lee, F., Mulligan, R., Berg, P., & Ringold, G. (1981) · Nature · doi:10.1038/294228a0 · Cited by 466
Self-citationHuman HSP70 promoter contains at least two distinct regulatory domains.
Wu, B. J., Kingston, R. E., & Morimoto, R. I. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.3.629 · Cited by 305
Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA
Huang, M. T. F., & Gorman, C. M. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.4.937 · Cited by 303
Metadata
- Authors
- Mulligan, R. C., & Berg, P.
- Venue
- Science
- Published
- 1980-09-19
- Volume
- 209
- Issue
- 4463
- Pages
- 1422-1427
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1980651107
- Cited by
- 738
- References
- 0
- FWCI
- 24.92
- Type
- journal article
- Language
- en
- Open access
- closed
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- Mulligan, R. C., & Berg, P. (1980). Expression of a Bacterial Gene in Mammalian Cells. Science. https://doi.org/10.1126/science.6251549
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
