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Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by n -Propyl Gallate
Abstract
n -Propyl gallate (0.1 to 0.25 molar, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated antibodies. Hence, prolonged photographic exposure of immunofluorescently labeled cells in the fluorescence microscope yields images with increased sensitivity, making feasible multiple data collection, as with serial optical sectioning.
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Cited by
Chapter 13 Fluorescence Microscopy in Three Dimensions
Agard, D. A., Hiraoka, Y., Shaw, P., & Sedat, J. W. (1989) · Methods in Cell Biology · doi:10.1016/s0091-679x(08)60986-3 · Cited by 649
Self-citationImmunocytochemical localization of two retinoid-binding proteins in vertebrate retina.
Bunt-Milam, A. H., & Saari, J. C. (1983) · The Journal of cell biology · doi:10.1083/jcb.97.3.703 · Cited by 455
Berryman, M., Franck, Z., & Bretscher, A. (1993) · Journal of Cell Science · doi:10.1242/jcs.105.4.1025 · Cited by 429
Current concepts in neuroanatomical tracing
Köbbert, C., Apps, R., Bechmann, I., Lanciego, J. L., Mey, J., & Thanos, S. (2000) · Progress in Neurobiology · doi:10.1016/s0301-0082(00)00019-8 · Cited by 417
Mjaatvedt, C. H., Lepera, R. C., & Markwald, R. R. (1987) · Developmental Biology · doi:10.1016/0012-1606(87)90206-5 · Cited by 157
Metadata
- Authors
- Giloh, H., & Sedat, J. W.
- Venue
- Science
- Published
- 1982-09-24
- Volume
- 217
- Issue
- 4566
- Pages
- 1252-1255
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1992644378
- Cited by
- 838
- References
- 0
- FWCI
- 43.35
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by n -Propyl Gallate. https://app.cochat.ai/papers/10.1126/science.7112126
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- Giloh, H., & Sedat, J. W. (1982). Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by n -Propyl Gallate. Science. https://doi.org/10.1126/science.7112126
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
