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High-Resolution Molecular Discrimination by RNA
Abstract
Species of RNA that bind with high affinity and specificity to the bronchodilator theophylline were identified by selection from an oligonucleotide library. One RNA molecule binds to theophylline with a dissociation constant K d of 0.1 μM. This binding affinity is 10,000-fold greater than the RNA molecule's affinity for caffeine, which differs from theophylline only by a methyl group at nitrogen atom N-7. Analysis by nuclear magnetic resonance indicates that this RNA molecule undergoes a significant change in its conformation or dynamics upon theophylline binding. Binding studies of compounds chemically related to theophylline have revealed structural features required for the observed binding specificity. These results demonstrate the ability of RNA molecules to exhibit an extremely high degree of ligand recognition and discrimination.
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Cited by
Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery
Gold, L., Ayers, D., Bertino, J., Bock, C., Bock, A., Brody, E. N., Carter, J., Dalby, A. B., Eaton, B. E., Fitzwater, T., et al. (2010) · PLoS ONE · doi:10.1371/journal.pone.0015004 · Cited by 1,754
“We believe that SOMAmers will be highly specific, given our previous experience with highly-specific aptamers including, for example, the drug Pegaptanib (Macugen) for the treatment age-related macular degeneration, which binds specifically to VEGF121 but not VEGF165 [37,38], and an aptamer that distinguishes theophyl…”
BackgroundAdaptive Recognition by Nucleic Acid Aptamers
Hermann, T., & Patel, D. J. (2000) · Science · doi:10.1126/science.287.5454.820 · Cited by 1,650
Applications of Aptamers as Sensors
Cho, E. J., Lee, J.-W., & Ellington, A. D. (2009) · Annual Review of Analytical Chemistry · doi:10.1146/annurev.anchem.1.031207.112851 · Cited by 788
Aptamers as therapeutic and diagnostic agents
Brody, E. N., & Gold, L. (2000) · Reviews in Molecular Biotechnology · doi:10.1016/s1389-0352(99)00004-5 · Cited by 494
SELEX: Critical factors and optimization strategies for successful aptamer selection
Kohlberger, M., & Gadermaier, G. (2022) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.2244 · Cited by 341
Metadata
- Authors
- Jenison, R. D., Gill, S. C., Pardi, A., & Polisky, B.
- Venue
- Science
- Published
- 1994-03-11
- Volume
- 263
- Issue
- 5152
- Pages
- 1425-1429
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2001266427
- Cited by
- 1,188
- References
- 0
- FWCI
- 12.13
- Type
- journal article
- Language
- en
- Open access
- closed
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- Jenison, R. D., Gill, S. C., Pardi, A., & Polisky, B. (1994). High-Resolution Molecular Discrimination by RNA. Science. https://doi.org/10.1126/science.7510417
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
