Inhibition by anions of human red-cell carbonic anhydrase-B – physiological and biochemical implications
Abstract
The hydration rate of CO 2 catalyzed by human red cell carbonic anhydrase B is 92 percent reduced by the normal concentrations of chloride and bicarbonate in red cells. This reflects a general sensitivity of this reaction to halides and other anions, up to 87 times greater than the effect on red cell carbonic anhydrase C. The catalytic hydration of CO 2 is generally more (up to 24 times) sensitive to inhibition by anions and sulfonamides than the dehydration of HCO 3 -, probably reflecting different mechanisms. The sensitivity of enzyme B to anion inhibition also depends upon the substrate, being much greater for CO 2 than for certain esters. On the basis of the very low catalytic activity of B for CO 2 in the presence of physiological concentration of chloride, and the fact that carbonic anhydrase C is effective for CO 2 hydration (in the presence of chloride) at a rate 340 times greater than that of CO 2 output from tissues, it appears that the biological role of enzyme B is not that of a carbonic anhydrase.
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The affinities of CA for carbon dioxide and bicarbonate are low enough that they are not likely to inhibit reactivity of CA with nitrite.
“The affinities of CA for carbon dioxide and bicarbonate are low enough that they are not likely to inhibit reactivity of CA with nitrite [103–105].”
From Mechanisms of nitrite bioactivation (2014) · cites this paper as [105]
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References 20 · Cited by 97
- Authors
- Maren, T. H., Rayburn, C. S., & Liddell, N. E.
- Affiliations
- University of Florida Health (US)
- Venue
- Science
- Published
- 1976-02-06
- Volume
- 191
- Issue
- 4226
- Pages
- 469-472
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W4240890892
- Cited by
- 97
- References
- 20
- Citations by year
- 2026: 1 · 2023: 1 · 2021: 1 · 2017: 1 · 2015: 3 · 2014: 1 · 2013: 1
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme function and inhibition
- Physical Sciences › Chemistry › Organic Chemistry › Chemical Reaction Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- carbonic anhydrase B, carbonic anhydrase C, anion inhibition, chloride, bicarbonate, CO2 hydration, CO2 dehydration, sulfonamide inhibition, erythrocytes, enzyme substrate specificity, enzyme activity
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