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Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase
Abstract
The natural arrest of vertebrate unfertilized eggs in second meiotic metaphase results from the activity of cytostatic factor (CSF). The product of the c-mos(xe) proto-oncogene is thought to be a component of CSF and can induce metaphase arrest when injected into blastomeres of two-cell embryos. The c-Mos(xe) protein can directly activate the mitogen-activated protein kinase kinase (MAP kinase kinase) in vitro, leading to activation of MAP kinase. MAP kinase and c-Mos(xe) are active in unfertilized eggs and are rapidly inactivated after fertilization. Microinjection of thiophosphorylated MAP kinase into one blastomere of a two-cell embryo induced metaphase arrest similar to that induced by c-Mos(xe). However, only arrest with c-Mos(xe) was associated with activation of endogenous MAP kinase. These results indicate that active MAP kinase is a component of CSF in Xenopus and suggest that the CSF activity of c-Mos(xe) is mediated by MAP kinase.
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Cited by
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Hunter, T., & Pines, J. (1994) · Cell · doi:10.1016/0092-8674(94)90543-6 · Cited by 2,091
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(1998) · Advances in Cancer Research · doi:10.1016/s0065-230x(08)60765-4 · Cited by 1,759
Ultrasensitivity in the mitogen-activated protein kinase cascade.
Huang, C. Y., & Ferrell, J. E. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.19.10078 · Cited by 1,186
Verlhac, M.-H., Kubiak, J. Z., Weber, M., Géraud, G., Colledge, W. H., Evans, M. J., & Maro, B. (1996) · Development · doi:10.1242/dev.122.3.815 · Cited by 320
Xenopus oocyte maturation: new lessons from a good egg
Ferrell, J. E. (1999) · BioEssays · doi:10.1002/(sici)1521-1878(199910)21:10<833::aid-bies5>3.0.co;2-p · Cited by 289
Metadata
- Authors
- Haccard, O., Sarcevic, B., Lewellyn, A., Hartley, R., Roy, L., Izumi, T., Erikson, E., & Maller, J.
- Venue
- Science
- Published
- 1993-11-19
- Volume
- 262
- Issue
- 5137
- Pages
- 1262-1265
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2081128958
- Cited by
- 261
- References
- 0
- FWCI
- 9.79
- Type
- journal article
- Language
- en
- Open access
- closed
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- Haccard, O., Sarcevic, B., Lewellyn, A., Hartley, R., Roy, L., Izumi, T., Erikson, E., & Maller, J. (1993). Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase. Science. https://doi.org/10.1126/science.8235656
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