The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Sensing structural intermediates in bacterial flagellar assembly by export of a negative regulator
Abstract
The ability of a regulatory protein to sense the integrity of the bacterial flagellar structure was investigated. In response to a defective hook-basal body complex, the anti-sigma 28 FlgM protein inhibits flagellin transcription. In cells with a functional hook-basal body complex, the flagellin genes are transcribed normally and the FlgM protein is expelled into the growth medium. In strains with a defective hook-basal body structure, FlgM is absent from the media. The presence of flagellin protein in the media is substantially reduced in strains carrying a FlgM-LacZ protein fusion, suggesting that the fusion is blocking the flagellar export apparatus. These results suggest that the FlgM protein assesses the integrity of the flagellar hook-basal body complex by itself being a substrate for export by the flagellar-specific export apparatus.
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Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Cited by
The Rotary Motor of Bacterial Flagella
Berg, H. C. (2003) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.72.121801.161737 · Cited by 1,457
Showing your ID: intrinsic disorder as an ID for recognition, regulation and cell signaling
Uversky, V. N., Oldfield, C. J., & Dunker, A. K. (2005) · Journal of Molecular Recognition · doi:10.1002/jmr.747 · Cited by 847
Regulation cascade of flagellar expression in Gram-negative bacteria
Soutourina, O. A., & Bertin, P. N. (2003) · FEMS Microbiology Reviews · doi:10.1016/s0168-6445(03)00064-0 · Cited by 363
FlgM gains structure in living cells
Dedmon, M. M., Patel, C. N., Young, G. B., & Pielak, G. J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.202331299 · Cited by 317
Jishage, M., Iwata, A., Ueda, S., & Ishihama, A. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.18.5447-5451.1996 · Cited by 314
Metadata
- Authors
- Hughes, K., Gillen, K., Semon, M., & Karlinsey, J.
- Venue
- Science
- Published
- 1993-11-19
- Volume
- 262
- Issue
- 5137
- Pages
- 1277-1280
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1996151076
- Cited by
- 484
- References
- 1
- FWCI
- 11.33
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hughes, K., Gillen, K., Semon, M., & Karlinsey, J. (1993). Sensing structural intermediates in bacterial flagellar assembly by export of a negative regulator. Science. https://doi.org/10.1126/science.8235660
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
