The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Requirement for a GTPase-Activating Protein in Vesicle Budding from the Endoplasmic Reticulum
Abstract
The binding and hydrolysis of guanosine triphosphate (GTP) by the small GTP-binding protein Sar1p is required to form transport vesicles from the endoplasmic reticulum (ER) in Saccharomyces cerevisiae . Experiments revealed that an interaction between Sar1p and the Sec23p subunit of an oligomeric protein is also required for vesicle budding. The isolated Sec23p subunit and the oligomeric complex stimulated guanosine triphosphatase (GTPase) activity of Sar1p 10- to 15-fold but did not activate two other small GTP-binding proteins involved in vesicle traffic (Ypt1p and ARF). Activation of GTPase was inhibited by an antibody to Sec23p but not by an antibody that inhibits the budding activity of the other subunit of the Sec23p complex. Also, activation was thermolabile in pure samples of Sec23p that were isolated from two independent sec23 mutant strains. It appears that Sec23p represents a new class of GTPase-activating protein because its sequence shows no similarity to any known member of this family.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The GTPase superfamily: conserved structure and molecular mechanism
Bourne, H. R., Sanders, D. A., & McCormick, F. (1991) · Nature · doi:10.1038/349117a0 · Cited by 3,392
The neurofibromatosis type 1 gene encodes a protein related to GAP
Xu, G., O'Connell, P., Viskochil, D., Cawthon, R., Robertson, M., Culver, M., Dunn, D., Stevens, J., Gesteland, R., White, R., et al. (1990) · Cell · doi:10.1016/0092-8674(90)90024-9 · Cited by 1,095
Cited by
BI-DIRECTIONAL PROTEIN TRANSPORT BETWEEN THE ER AND GOLGI
Lee, M. C. S., Miller, E. A., Goldberg, J., Orci, L., & Schekman, R. (2004) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.20.010403.105307 · Cited by 813
Self-citationOrganization of the ER–Golgi interface for membrane traffic control
Brandizzi, F., & Barlowe, C. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3588 · Cited by 522
Self-citationSeaman, M. N. J., Harbour, M. E., Tattersall, D., Read, E., & Bright, N. (2009) · Journal of Cell Science · doi:10.1242/jcs.048686 · Cited by 375
Dynamics of the COPII coat with GTP and stable analogues
Antonny, B., Madden, D., Hamamoto, S., Orci, L., & Schekman, R. (2001) · Nature Cell Biology · doi:10.1038/35078500 · Cited by 314
Self-citationStructural and mechanistic insights into regulation of the retromer coat by TBC1d5
Jia, D., Zhang, J.-S., Li, F., Wang, J., Deng, Z., White, M. A., Osborne, D. G., Phillips-Krawczak, C., Gomez, T. S., Li, H., et al. (2016) · Nature Communications · doi:10.1038/ncomms13305 · Cited by 118
Metadata
- Authors
- Yoshihisa, T., Barlowe, C., & Schekman, R.
- Venue
- Science
- Published
- 1993-03-05
- Volume
- 259
- Issue
- 5100
- Pages
- 1466-1468
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1990364715
- Cited by
- 384
- References
- 2
- FWCI
- 6.35
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Requirement for a GTPase-Activating Protein in Vesicle Budding from the Endoplasmic Reticulum. https://app.cochat.ai/papers/10.1126/science.8451644
- The paper
- Yoshihisa, T., Barlowe, C., & Schekman, R. (1993). Requirement for a GTPase-Activating Protein in Vesicle Budding from the Endoplasmic Reticulum. Science. https://doi.org/10.1126/science.8451644
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
