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Altered Growth of Human Colon Cancer Cell Lines Disrupted at Activated Ki- ras
Abstract
Point mutations that activate the Ki- ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki- ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki- ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c- myc . Thus, the activated Ki- ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.
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Metadata
- Authors
- Shirasawa, S., Furuse, M., Yokoyama, N., & Sasazuki, T.
- Venue
- Science
- Published
- 1993-04-02
- Volume
- 260
- Issue
- 5104
- Pages
- 85-88
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2027737365
- Cited by
- 620
- References
- 0
- FWCI
- 6.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- Shirasawa, S., Furuse, M., Yokoyama, N., & Sasazuki, T. (1993). Altered Growth of Human Colon Cancer Cell Lines Disrupted at Activated Ki- ras. Science. https://doi.org/10.1126/science.8465203
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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