Long-term functional maintenance of primary human hepatocytes in vitro
Abstract
Keep hepatocytes fresh: Freshly isolated primary human hepatocytes (PHHs) quickly lose their identity and function in vitro, limiting their application in modeling infectious liver diseases and screening drugs. Xiang et al. describe a simple and effective five-chemical culture condition that can maintain the mature function of cultured PHHs for an extended period of time. PHHs cultured in this way can support the entire viral life cycle and recapitulate long-term infection of the hepatitis B virus. This system created a useful drug-screening platform for developing new antiviral strategies. Science , this issue p. 399
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MPH dedifferentiation can only be partially inhibited by combined inhibition of multiple dedifferentiating signaling pathways or by forced expression of supra-physiologic amounts of HNF4A.
“previous reports indicated that MPH dedifferentiation could only be partially inhibited when using combined inhibition of multiple dedifferentiating signaling pathways (Sun et al, 2019; Xiang et al, 2019) or through forced expression of supra‐physiologic amounts of the Hep‐ID TF HNF4A (Nishikawa et al, 2015; Tafaleng et al, 2021; Yang et al, 2021).”
From An extended transcription factor regulatory network controls hepatocyte identity (2023) · cites this paper as Xiang et al, 2019
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References 18 · Cited by 223
- Authors
- Xiang, C., Du, Y., Meng, G., Soon Yi, L., Sun, S., Song, N., Zhang, X., Xiao, Y., Wang, J., Yi, Z., Liu, Y., Xie, B., Wu, M., Shu, J., Sun, D., Jia, J., Liang, Z., Sun, D., Huang, Y., Shi, Y., Xu, J., Lu, F., Li, C., Xiang, K., Yuan, Z., Lu, S., & Deng, H.
- Affiliations
- Peking University (CN)
- School of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
- Shanghai Public Health Clinical Center (CN)
- Shanghai Medical College of Fudan University (CN)
- Beijing YouAn Hospital (CN)
- Chinese PLA General Hospital (CN)
- Corresponding author
- Xiang, Chengang
- Du, Yuanyuan
- Meng, Gaofan
- Yuan, Zhenghong
- Lu, Shichun
- Deng, Hongkui
- ORCID
- Venue
- Science
- Published
- 2019-04-26
- Volume
- 364
- Issue
- 6438
- Pages
- 399-402
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2941187779
- Cited by
- 223
- References
- 18
- Citations by year
- 2026: 20 · 2025: 35 · 2024: 26 · 2023: 35 · 2022: 35 · 2021: 34 · 2020: 27 · 2019: 11
- FWCI
- 20.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Hepatitis B Virus Studies
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- primary human hepatocytes, hepatitis B, hepatocyte gene expression, antiviral drug screening, hepatocyte differentiation
- MeSH terms
- Antiviral Agents, DNA, Circular, DNA, Viral, Drug Evaluation, Preclinical, Hepatitis B virus, Humans, Virion, Virus Cultivation, Hepatocytes, Transcriptome, Primary Cell Culture
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