Pharmacological inhibition of a microRNA family in nonhuman primates by a seed-targeting 8-mer antimiR
Open accessAbstract
Long-term treatment of obese, insulin-resistant nonhuman primates with a seed-targeting antimiR oligonucleotide against the microRNA-33 family derepresses hepatic expression of miR-33 targets, increases circulating HDL cholesterol, and has a clean safety profile.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Antagonism of miR-33 can also increase circulating levels of HDL-C in non-human primates.
“Importantly, studies performed in monkeys have demonstrated that antagonism of miR-33 can also increase circulating levels of HDL-C in non-human primates (Rayner et al., 2011a; Rottiers et al., 2013).”
From Genetic dissection of the impact of miR-33a and miR-33b during the progression of atherosclerosis (2017) · cites this paper as Rottiers et al., 2013
Findings in non-human primates suggest that miR-33a and miR-33b play crucial roles in cholesterol regulation in humans.
“These findings have also been validated in non-human primates, suggesting that miR-33a and miR-33b play crucial roles in cholesterol regulation in humans 17 , 18) .”
From Critical Role of microRNA-33a/b in Cardiovascular and Metabolic Disease: Molecular Mechanisms and Therapeutic Perspectives (2026) · cites this paper as [18]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
No citation lists for this paper
No citation lists for this paper yet — checked again daily.
References 34 · Cited by 121
Research works printed but not linked: 28
- [1]Ambros V The functions of animal microRNAs. Nature 2004 431 350 355 15372042
- [2]Bartel DP MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004 116 281 297 14744438
- [3]Bartel DP MicroRNAs: target recognition and regulatory functions. Cell 2009 136 215 233 19167326 PMC3794896
- [4]Hertel J Lindemeyer M Missal K Fried C Tanzer A Flamm C Hofacker IL Stadler PF The expansion of the metazoan microRNA repertoire. BMC Genomics 2006 7 25 16480513 PMC1388199
- [5]Lewis BP Burge CB Bartel DP Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 2005 120 15 20 15652477
- [6]Mendell JT Olson EN MicroRNAs in stress signaling and human disease. Cell 2012 148 1172 1187 22424228 PMC3308137
- [7]Lanford RE Hildebrandt-Eriksen ES Petri A Persson R Lindow M Munk ME Kauppinen S Orum H Therapeutic silencing of microRNA-122 in primates with chronic hepatitis C virus infection. Science 2010 327 198 201 19965718 PMC3436126
- [8]Janssen HL Reesink HW Lawitz EJ Zeuzem S Rodriguez-Torres M Patel K van der Meer AJ Patick AK Chen A Zhou Y Persson R King BD Kauppinen S Levin AA Hodges MR Treatment of HCV infection by targeting microRNA. N Engl J Med 2013 368 1685 1694 23534542
- [9]Grimson A Farh KK Johnston WK Garrett-Engele P Lim LP Bartel DP MicroRNA targeting specificity in mammals: determinants beyond seed pairing. Molecular cell 2007 27 91 105 17612493 PMC3800283
- [10]Obad S dos Santos CO Petri A Heidenblad M Broom O Ruse C Fu C Lindow M Stenvang J Straarup EM Hansen HF Koch T Pappin D Hannon GJ Kauppinen S Silencing of microRNA families by seed-targeting tiny LNAs. Nat Genet 2011 43 371 378 21423181 PMC3541685
- [11]Zhang Y Roccaro AM Rombaoa C Flores L Obad S Fernandes SM Sacco A Liu Y Ngo H Quang P Azab AK Azab F Maiso P Reagan M Brown JR Thai TH Kauppinen S Ghobrial IM LNA-mediated anti-miR-155 silencing in low-grade B-cell lymphomas. Blood 2012 120 1678 1686 22797699
- [12]Leucci E Zriwil A Gregersen LH Jensen KT Obad S Bellan C Leoncini L Kauppinen S Lund AH Inhibition of miR-9 de-represses HuR and DICER1 and impairs Hodgkin lymphoma tumour outgrowth in vivo. Oncogene 2012 22310293
- [13]Bernardo BC Gao XM Winbanks CE Boey EJ Tham YK Kiriazis H Gregorevic P Obad S Kauppinen S Du XJ Lin RC McMullen JR Therapeutic inhibition of the miR-34 family attenuates pathological cardiac remodeling and improves heart function. Proc Natl Acad Sci U S A 2012 109 17615 17620 23047694 PMC3491509
- [15]Rayner KJ Suarez Y Davalos A Parathath S Fitzgerald ML Tamehiro N Fisher EA Moore KJ Fernandez-Hernando C MiR-33 contributes to the regulation of cholesterol homeostasis. Science 2010 328 1570 1573 20466885 PMC3114628
- [16]Marquart TJ Allen RM Ory DS Baldan A miR-33 links SREBP-2 induction to repression of sterol transporters. Proc Natl Acad Sci U S A 2010 107 12228 12232 20566875 PMC2901433
- [17]Horie T Ono K Horiguchi M Nishi H Nakamura T Nagao K Kinoshita M Kuwabara Y Marusawa H Iwanaga Y Hasegawa K Yokode M Kimura T Kita T MicroRNA-33 encoded by an intron of sterol regulatory element-binding protein 2 (Srebp2) regulates HDL in vivo. Proc Natl Acad Sci U S A 2010 107 17321 17326 20855588 PMC2951399
- [21]Rayner KJ Esau CC Hussain FN McDaniel AL Marshall SM van Gils JM Ray TD Sheedy FJ Goedeke L Liu X Khatsenko OG Kaimal V Lees CJ Fernandez-Hernando C Fisher EA Temel RE Moore KJ Inhibition of miR-33a/b in non-human primates raises plasma HDL and lowers VLDL triglycerides. Nature 2011 478 404 407 22012398 PMC3235584
- [22]Attie AD ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends Biochem Sci 2007 32 172 179 17324574
- [23]Rader DJ Tall AR The not-so-simple HDL story: Is it time to revise the HDL cholesterol hypothesis? Nat Med 2012 18 1344 1346 22961164
- [24]Horie T Osamu B Kuwabara Y Chujo Y Watanabe S Kinoshita M Horiguchi M Nakamura T Chonabayashi K Hishizawa M Hasegawa K Kume N Yokode M Kita T Kimura T Ono K MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE−/− Mice. JAMA 2012 PMC3540673 23316322
- [26]Rottiers V Najafi-Shoushtari SH Kristo F Gurumurthy S Zhong L Li Y Cohen DE Gerszten RE Bardeesy N Mostoslavsky R Naar AM MicroRNAs in Metabolism and Metabolic Diseases. Cold Spring Harb Symp Quant Biol 2011 PMC3880782 22156303
- [27]Liddie S Goody RJ Valles R Lawrence MS Clinical chemistry and hematology values in a Caribbean population of African green monkeys. J Med Primatol 2010 39 389 398 20524957
- [28]Avramoglu RK Basciano H Adeli K Lipid and lipoprotein dysregulation in insulin resistant states. Clin Chim Acta 2006 368 1 19 16480697
- [29]Straarup EM Fisker N Hedtjarn M Lindholm MW Rosenbohm C Aarup V Hansen HF Orum H Hansen JB Koch T Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B mRNA and serum cholesterol in mice and non-human primates. Nucleic Acids Res 2010 38 7100 7111 20615897 PMC2978335
- [30]Cann JA Kavanagh K Jorgensen MJ Mohanan S Howard TD Gray SB Hawkins GA Fairbanks LA Wagner JD Clinicopathologic characterization of naturally occurring diabetes mellitus in vervet monkeys. Vet Pathol 2010 47 713 718 20460450
- [31]Chen G Liang G Ou J Goldstein JL Brown MS Central role for liver X receptor in insulin-mediated activation of Srebp-1c transcription and stimulation of fatty acid synthesis in liver. Proc Natl Acad Sci U S A 2004 101 11245 11250 15266058 PMC509189
- [32]Rotllan N Ramirez CM Aryal B Esau CC Fernandez-Hernando C Therapeutic Silencing of MicroRNA-33 Inhibits the Progression of Atherosclerosis in Ldlr-/- Mice. Arterioscler Thromb Vasc Biol 2013 PMC4157595 23702658
- [34]Elmen J Lindow M Silahtaroglu A Bak M Christensen M Lind-Thomsen A Hedtjarn M Hansen JB Hansen HF Straarup EM McCullagh K Kearney P Kauppinen S Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver. Nucleic Acids Res 2008 36 1153 1162 18158304 PMC2275095
- Authors
- Rottiers, V., Obad, S., Petri, A., McGarrah, R., Lindholm, M. W., Black, J. C., Sinha, S., Goody, R. J., Lawrence, M. S., deLemos, A. S., Hansen, H. F., Whittaker, S., Henry, S., Brookes, R., Najafi-Shoushtari, S. H., Chung, R. T., Whetstine, J. R., Gerszten, R. E., Kauppinen, S., & Näär, A. M.
- Affiliations
- Harvard University (US)
- Danish National Metrology Institute (DK)
- Aalborg University Hospital (DK)
- RxGen Inc., 100 Deepwood Drive, Hamden, CT 06517, USA.
- Corresponding author
- Rottiers, Veerle
- Obad, Susanna
- Kauppinen, Sakari
- Näär, Anders M.
- ORCID
- Venue
- Science Translational Medicine
- Published
- 2013-11-20
- Volume
- 5
- Issue
- 212
- Pages
- 212ra162-212ra162
- ISSN
- 1946-6234 (ISSN-L)
- 1946-6242
- PMC
- PMC4033576
- OpenAlex
- W2102842031
- Cited by
- 121
- References
- 34
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 2 · 2023: 7 · 2022: 8 · 2021: 12 · 2020: 6 · 2019: 4 · 2018: 10 · 2017: 10 · 2016: 18 · 2015: 18 · 2014: 20
- FWCI
- 6.57
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science Translational Medicine (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- VBN Forskningsportal (Aalborg Universitet) (submittedVersion)
- Funders
- Lundbeckfonden (R151-2013-14476)
- Novo Nordisk Fonden (NNF13OC0006395)
- National Cancer Institute (CA059267, C06CA059267)
- National Institute of General Medical Sciences (R01GM097360)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK093216-01A1, F32DK093216, R21DK084459, R01DK094184, DK078772, K24DK078772, P30DK043351)
- National Institute of Neurological Disorders and Stroke (#NS072030, P30NS072030)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- microRNA inhibition, miR-33, non-human primates, obesity, insulin resistance, HDL cholesterol, ABCA1, protein synthesis inhibition, drug safety and efficacy
- MeSH terms
- Animals, Female, Humans, Cholesterol, HDL, Mice, Inbred C57BL, Primates, Gene Silencing, MicroRNAs, Mice, Hep G2 Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
