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Fanciful FRET
Abstract
The validity of experiments based on Förster resonance energy transfer (FRET), an imaging technique widely used to measure protein-protein interactions in living cells, critically depends on the accurate and precise measurement of FRET efficiency. The use of FRET standards to determine FRET efficiency, and a consideration of such factors as how the abundance of FRET acceptors and the stoichiometry of donors and acceptors in a molecular complex can affect measured FRET efficiency, will enhance the usefulness with which FRET experiments can be interpreted.
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Cited by
Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues
Chudakov, D. M., Matz, M. V., Lukyanov, S., & Lukyanov, K. A. (2010) · Physiological Reviews · doi:10.1152/physrev.00038.2009 · Cited by 1,375
Fluorescence lifetime imaging – techniques and applications
BECKER, W. (2012) · Journal of Microscopy · doi:10.1111/j.1365-2818.2012.03618.x · Cited by 890
Fluorescent protein FRET: the good, the bad and the ugly
Piston, D. W., & Kremers, G.-J. (2007) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.08.003 · Cited by 849
Accuracy and precision in quantitative fluorescence microscopy
Waters, J. C. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200903097 · Cited by 613
Zhou, K., Liu, H., Zhang, S., Huang, X., Wang, Y., Huang, G., Sumer, B. D., & Gao, J. (2012) · Journal of the American Chemical Society · doi:10.1021/ja300176w · Cited by 344
Metadata
- Authors
- Vogel, S. S., Thaler, C., & Koushik, S. V.
- Venue
- Science's STKE
- Published
- 2006-04-18
- Volume
- 2006
- Issue
- 331
- Pages
- re2-re2
- ISSN
- 1525-8882 (ISSN-L)
- OpenAlex
- W4211238694
- Cited by
- 241
- References
- 0
- FWCI
- 9.98
- Type
- journal article
- Language
- en
- Open access
- closed
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
