Identification and Characterization of Four Azole-Resistant erg3 Mutants of Candida albicans

Martel, Claire M.; Parker, Josie E.; Bader, Oliver; Weig, Michael; Gross, Uwe; Warrilow, Andrew G. S.; Rolley, Nicola; Kelly, Diane E.; Kelly, Steven L.

doi:10.1128/aac.00348-10

Identification and Characterization of Four Azole-Resistant erg3 Mutants of Candida albicans

Sterol analysis identified four Candida albicans erg3 mutants in which ergosta 7,22-dienol, indicative of perturbations in sterol Δ 5,6 -desaturase (Erg3p) activity, comprised >5% of the total sterol fraction. The erg3 mutants (CA12, CA488, CA490, and CA1008) were all resistant to fluconazole, vo…