Fluorescence Microscopy Demonstrates Enhanced Targeting of Telavancin to the Division Septum of Staphylococcus aureus
Open accessAbstract
The cellular binding patterns of fluorescent conjugates of telavancin and vancomycin were evaluated in Staphylococcus aureus by fluorescence microscopy and ratio imaging analysis. Telavancin showed enhanced binding at the division septum compared to vancomycin. This result is consistent with observations that telavancin binds with higher affinity to lipid II than to d-Ala-d-Ala residues in the cell wall, thus demonstrating the preferential binding of telavancin to the site of active cell wall biosynthesis.
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Stationary growth-phase conditions make it less likely that bacterial cell wall-active antibiotics (β-lactams and glycopeptides) are optimally effective.
“In addition, the stationary growth-phase conditions make it less likely that bacterial cell wall-active antibiotics (β-lactams and glycopeptides) are optimally effective.[77][78][79]”
From Infective Endocarditis in Adults: Diagnosis, Antimicrobial Therapy, and Management of Complications: A Scientific Statement for Healthcare Professionals From the American Heart Association (2015) · cites this paper as [77]
The passage labeled Abstract (page unavailable) says glycopeptides must reach the site of active cell-wall biosynthesis to act on their targets. It does not address stationary-phase conditions, reduced antibiotic effectiveness during that phase, or β-lactams. (LLM, conf=0.78)
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References 17 · Cited by 32
Research works printed but not linked: 2
- [1]Breukink, E., P. P. A. Humphrey, B. M. Benton, and I. Visscher. 2006. Evidence for a multivalent interaction between telavancin and membrane-bound lipid II, abstr. C1-678. Abstr. 46th Intersc. Conf. Antimicrob. Agents Chemother., San Francisco, CA.
- [11]Lunde, C. S., C. H. Rexer, S. R. Hartouni, S. Axt, and B. M. Benton. 2009. Telavancin targets the division septum of Staphylococcus aureus: analysis of cellular binding by fluorescence microscopy, abstr. C1-1343. Abstr. 49th Intersci. Conf. Antimicrob. Agents Chemother., San Francisco, CA. [DOI] [PMC free article] [PubMed]
- Authors
- Lunde, C. S., Rexer, C. H., Hartouni, S. R., Axt, S., & Benton, B. M.
- Affiliations
- Theravance Biopharma (United States) (US)
- Corresponding author
- Lunde, Christopher S.
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2010-05
- Volume
- 54
- Issue
- 5
- Pages
- 2198-2200
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC2863671
- OpenAlex
- W2132629057
- Cited by
- 32
- References
- 17
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 5 · 2014: 2 · 2013: 5 · 2012: 6
- FWCI
- 3.11
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Keywords
- telavancin, vancomycin, Staphylococcus aureus, cell division septum, lipid II, D-Ala-D-Ala, cell wall biosynthesis, antibiotic binding sites, glycopeptides
- MeSH terms
- Lipoglycopeptides, Aminoglycosides, Anti-Bacterial Agents, Cell Division, Cell Wall, Fluorescent Antibody Technique, Microscopy, Fluorescence, Vancomycin, Methicillin-Resistant Staphylococcus aureus
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