Effect of Antibiotic Treatment on the Intestinal Metabolome
Open accessAbstract
The importance of the mammalian intestinal microbiota to human health has been intensely studied over the past few years. It is now clear that the interactions between human hosts and their associated microbial communities need to be characterized in molecular detail if we are to truly understand human physiology. Additionally, the study of such host-microbe interactions is likely to provide us with new strategies to manipulate these complex systems to maintain or restore homeostasis in order to prevent or cure pathological states. Here, we describe the use of high-throughput metabolomics to shed light on the interactions between the intestinal microbiota and the host. We show that antibiotic treatment disrupts intestinal homeostasis and has a profound impact on the intestinal metabolome, affecting the levels of over 87% of all metabolites detected. Many metabolic pathways that are critical for host physiology were affected, including bile acid, eicosanoid, and steroid hormone synthesis. Dissecting the molecular mechanisms involved in the impact of beneficial microbes on some of these pathways will be instrumental in understanding the interplay between the host and its complex resident microbiota and may aid in the design of new therapeutic strategies that target these interactions.
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Antibiotics represent the most direct and effective way of targeting the gut microbiota.
“antibiotics represent the most direct and effective way of targeting the gut microbiota (Antunes et al., 2011;Jakobsson et al., 2010)”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Antunes et al., 2011
The paper’s introductory text (page unavailable) says antibiotics have significant effects on the intestinal microbiota, supporting their use to perturb it. Neither that passage nor the Results passage comparing changes over time (page unavailable) establishes that antibiotics are the most direct and effective approach relative to other ways of targeting the microbiota. (LLM, conf=0.85)
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References 34 · Cited by 271
Research works printed but not linked: 3
- [11]11. Leroy, Q., and D. Raoult. 2010. Review of microarray studies for hostintracellular pathogen interactions. J. Microbiol. Methods 81: 81-95.
- [26]26. Shimada, K., K. S. Bricknell, and S. M. Finegold. 1969. Deconjugation of bile acids by intestinal bacteria: review of literature and additional studies. J. Infect. Dis. 119: 73-81.
- [29]29. Thompson, L. J., et al. 2009. Transcriptional response in the peripheral blood of patients infected with Salmonella enterica serovar Typhi. Proc. Natl. Acad. Sci. U. S. A. 106: 22433-22438.
- Authors
- Antunes, L. C. M., Han, J., Ferreira, R. B. R., Lolić, P., Borchers, C. H., & Finlay, B. B.
- Affiliations
- University of British Columbia (CA)
- University of Victoria (CA)
- Michael Smith Laboratories, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
- Corresponding author
- Finlay, B. Brett
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2011-04
- Volume
- 55
- Issue
- 4
- Pages
- 1494-1503
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC3067180
- OpenAlex
- W2149529467
- Cited by
- 271
- References
- 34
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 15 · 2023: 10 · 2022: 20 · 2021: 23 · 2020: 18 · 2019: 18 · 2018: 22 · 2017: 20 · 2016: 23 · 2015: 23 · 2014: 19 · 2013: 22 · 2012: 13
- FWCI
- 8.06
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- Genome Canada
- Crohn's and Colitis Foundation
- Crohn's and Colitis Foundation of Canada
- Genome British Columbia
- University of British Columbia
- Canadian Institutes of Health Research
- Foreign Affairs and International Trade Canada
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- antibiotic therapy, gut microbiota, intestinal metabolome, host-microbe interactions, intestinal homeostasis, bile acid synthesis, eicosanoid biosynthesis, steroidogenesis
- MeSH terms
- Animals, Anti-Bacterial Agents, Enzyme-Linked Immunosorbent Assay, Female, Intestinal Mucosa, Intestines, Male, Mice, Inbred C57BL, Mass Spectrometry, Streptomycin, Eicosanoids, Mice, Metabolome
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